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Published on August 24, 2007; 10.1104/pp.107.105742

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Received July 18, 2007
Accepted August 22, 2007

Post-Transcriptional Regulation of High-Affinity Sulfate Transporters in Arabidopsis by Sulfur Nutrition

Naoko Yoshimoto , Eri Inoue , Akiko Watanabe-Takahashi , Kazuki Saito , and Hideki Takahashi *

RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan; Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan

* Corresponding author; email: hideki{at}riken.jp.

High-affinity sulfate transporters, SULTR1;1 and SULTR1;2, are expressed at epidermis and cortex of Arabidopsis (Arabidopsis thaliana) roots during sulfur limitation. Here we report that SULTR1;1 and SULTR1;2 are the two essential components of sulfate uptake system in roots, and are regulated at post-transcriptional levels together with the previously reported transcriptional control. Double knockout of SULTR1;1 and SULTR1;2 by T-DNA insertion gene disruption resulted in complete lack of sulfate uptake capacity and severely affected the plant growth under low-sulfur conditions. Expression of epitope-tagged proteins, SULTR1;1mycHis and SULTR1;2mycHis, under the control of cauliflower mosaic virus 35S promoter rescued the uptake of sulfate and the growth of sultr1;1 sultr1;2 double knockout mutant. The recovery of the double knockout phenotypes was attributable to the post-transcriptional accumulation of sulfate transporter proteins that derive from the epitope-tagged transgenic constructs. Both SULTR1;1mycHis and SUTLR1;2mycHis mRNAs were predominantly found in roots, and slightly induced by long-term sulfur limitation. SULTR1;1mycHis and SULTR1;2mycHis proteins were found exclusively in roots, and significantly accumulated by sulfur limitation, correlating with the induction of sulfate uptake activities. In the time-course of short-term sulfate starvation treatment, SULTR1;1mycHis and SULTR1;2mycHis proteins were significantly accumulated during the 8-72 hr period causing substantial induction of sulfate uptake activities, while their corresponding mRNAs were expressed constantly around the initial levels except for the transient induction in the first 2 hr. The present study suggested the importance of root-specific and sulfur-deficiency inducible accumulation of SULTR1;1 and SULTR1;2 sulfate transporter proteins for the acquisition of sulfate from low-sulfur environment.




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H. Rouached, M. Wirtz, R. Alary, R. Hell, A. B. Arpat, J.-C. Davidian, P. Fourcroy, and P. Berthomieu
Differential Regulation of the Expression of Two High-Affinity Sulfate Transporters, SULTR1.1 and SULTR1.2, in Arabidopsis
Plant Physiology, June 1, 2008; 147(2): 897 - 911.
[Abstract] [Full Text] [PDF]


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