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Published on August 31, 2007; 10.1104/pp.107.106203

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Received July 25, 2007
Accepted August 22, 2007

Transcriptomic and Proteomic Analyses of Pericycle Cells of the Maize (Zea mays L.) Primary Root

Diana Dembinsky , Katrin Woll , Muhammad Saleem , Yan Liu , Yan Fu , Lisa A. Borsuk , Tobias Lamkemeyer , Claudia Fladerer , Johannes Madlung , Brad Barbazuk , Alfred Nordheim , Dan Nettleton , Patrick S. Schnable , and Frank Hochholdinger *

Center for Plant Molecular Biology, Department of General Genetics, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA; Department of Genetics, Development, and Cell Biology, Iowa State University, Ames IA 50011-3650, USA; Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA, USA; Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, 72076 Tuebingen, Germany; Department of Statistics, Iowa State University, Ames IA 50011, USA; Interdepartmental Genetics Major; Center for Plant Genomics, Iowa State University, Ames IA 50011-3650, USA

* Corresponding author; email: frank.hochholdinger{at}zmbp.uni-tuebingen.de.

Each plant cell type expresses a unique transcriptome and proteome at different stages of differentiation dependant on its developmental fate. This study compared gene expression and protein accumulation in cell-cycle-competent primary root pericycle cells of maize (Zea mays) prior to their first division and lateral root initiation. These are the only root cells that maintain the competence to divide after they leave the meristematic zone. Pericycle cells of the inbred line B73 were isolated via laser capture microdissection. Microarray experiments identified 32 genes preferentially expressed in pericycle versus all other root cells that have left the apical meristem; selective subtractive hybridization identified nine genes preferentially expressed in pericycle versus central cylinder cells of the same root region. Transcription and protein synthesis represented the most abundant functional categories among these pericycle specific genes. Moreover, 701 expressed sequence tags (ESTs) were generated from pericycle and central cylinder cells. Among those, transcripts related to protein synthesis and cell fate were significantly enriched in pericycle versus non-pericycle cells. In addition, 77 EST clusters not previously identified in maize EST or genomic databases were identified. Finally, among the most abundant soluble pericycle proteins separated via 2-dimensional electrophoresis, 20 proteins were identified via ESI MS/MS mass spectrometry, thus defining a first reference dataset of the maize pericycle proteome. Among those, two proteins were preferentially expressed in the pericycle. In summary, these pericycle specific gene expression experiments define the distinct molecular events during the specification of cell-cycle competent pericycle cells prior to their first division and demonstrate that pericycle specification and lateral root initiation might be controlled by a different set of genes.




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