Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 24, 2007; 10.1104/pp.107.106377

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
145/4/1232    most recent
pp.107.106377v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindbo, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindbo, J. A.
Agricola
Right arrow Articles by Lindbo, J. A.

Received July 28, 2007
Accepted August 16, 2007

TRBO: A High Efficiency Tobacco Mosaic Virus RNA Based Overexpression Vector

John A. Lindbo *

Department of Plant Pathology, Ohio State University/ Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA

* Corresponding author; email: John_Lindbo{at}yahoo.com.

Transient expression is a rapid, useful approach for producing proteins of interest in plants. Tobacco mosaic virus (TMV) -based transient expression vectors can express very high levels of foreign proteins in plants. However TMV vectors are, in general, not efficiently delivered to plant cells by agroinfection. It was determined that agroinfection was very efficient with a 35S promoter-driven TMV replicon that lacked the TMV coat protein (CP) gene sequence. This CP deletion vector had several useful features as a transient expression system including: improved ease of use, higher protein expression rates, and improved biocontainment. Using this TMV expression vector some foreign proteins were expressed at levels of 3 to 5 mg per g fresh weight of plant tissue. It is proposed that this new transient expression vector will be a useful tool for expressing recombinant proteins in plants for either research or production purposes.




This article has been cited by other articles:


Home page
 Plant CellHome page
S.-K. Oh, C. Young, M. Lee, R. Oliva, T. O. Bozkurt, L. M. Cano, J. Win, J. I.B. Bos, H.-Y. Liu, M. van Damme, et al.
In Planta Expression Screens of Phytophthora infestans RXLR Effectors Reveal Diverse Phenotypes, Including Activation of the Solanum bulbocastanum Disease Resistance Protein Rpi-blb2
PLANT CELL, September 1, 2009; 21(9): 2928 - 2947.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Song, J. Win, M. Tian, S. Schornack, F. Kaschani, M. Ilyas, R. A. L. van der Hoorn, and S. Kamoun
Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3
PNAS, February 3, 2009; 106(5): 1654 - 1659.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Sainsbury and G. P. Lomonossoff
Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication
Plant Physiology, November 1, 2008; 148(3): 1212 - 1218.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists