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Published on August 24, 2007; 10.1104/pp.107.106377

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Received July 28, 2007
Accepted August 16, 2007

TRBO: A High Efficiency Tobacco Mosaic Virus RNA Based Overexpression Vector

John A. Lindbo *

Department of Plant Pathology, Ohio State University/ Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA

* Corresponding author; email: John_Lindbo{at}yahoo.com.

Transient expression is a rapid, useful approach for producing proteins of interest in plants. Tobacco mosaic virus (TMV) -based transient expression vectors can express very high levels of foreign proteins in plants. However TMV vectors are, in general, not efficiently delivered to plant cells by agroinfection. It was determined that agroinfection was very efficient with a 35S promoter-driven TMV replicon that lacked the TMV coat protein (CP) gene sequence. This CP deletion vector had several useful features as a transient expression system including: improved ease of use, higher protein expression rates, and improved biocontainment. Using this TMV expression vector some foreign proteins were expressed at levels of 3 to 5 mg per g fresh weight of plant tissue. It is proposed that this new transient expression vector will be a useful tool for expressing recombinant proteins in plants for either research or production purposes.




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F. Sainsbury and G. P. Lomonossoff
Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication
Plant Physiology, November 1, 2008; 148(3): 1212 - 1218.
[Abstract] [Full Text] [PDF]


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