Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 26, 2007; 10.1104/pp.107.106526

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
145/4/1220    most recent
pp.107.106526v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verweire, D.
Right arrow Articles by Angenon, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verweire, D.
Right arrow Articles by Angenon, G.
Agricola
Right arrow Articles by Verweire, D.
Right arrow Articles by Angenon, G.

Received July 30, 2007
Accepted October 22, 2007

Marker-free transgenic plants through genetically programmed auto-excision

Dimitri Verweire , Kristof Verleyen , Sylvie De Buck , Martine Claeys , and Geert Angenon *

Laboratory of Plant Genetics, Institute for Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium; Department of Plant Systems Biology, Flanders Institute for Biotechnology, and Department of Molecular Genetics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium

* Corresponding author; email: Geert.Angenon{at}vub.ac.be.

We present here a vector-system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed. By introducing a "germline-specific auto-excision vector" (GSA vector) containing a cre recombinase gene under control of a germline-specific promoter transgenic plants become genetically programmed to lose the marker when its presence is not longer required, i.e. after the initial selection of primary transformants. By using promoters with different germline functionalities, two modules of this genetic program were developed. In the first module the promoter, placed upstream of the cre gene, confers CRE functionality in both the male and the female germline or in the common germline (e.g. floral meristem cells). In the second module a promoter conferring single-germline specific CRE functionality was introduced upstream of the cre gene. Promoter sequences used in the work presented here are derived from the APETALA1 and SOLO DANCERS genes from Arabidopsis thaliana Col-0 conferring common-germline and single-germline functionality, respectively. The introduction of the genetic program did not reduce the transformation efficiency. Marker-free homozygous progeny plants were efficiently obtained regardless of which promoter was used. In addition, simplification of complex transgene loci was observed.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists