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Published on October 11, 2007; 10.1104/pp.107.106633

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Received July 31, 2007
Accepted September 28, 2007

Novel Plant Transformation Vectors Containing the Super-promoter

Lan-Ying Lee , Maria E. Kononov , Burgund Bassuner , Bronwyn R. Frame , Kan Wang , and Stanton B. Gelvin *

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392; Center for Plant Transformation, Plant Science Institute, and Department of Agronomy, Ames, IA 50011-1010

* Corresponding author; email: gelvin{at}bilbo.bio.purdue.edu.

We developed novel plasmids and T-DNA binary vectors that incorporate a modified and more useful form of the super-promoter. The super-promoter consists of a trimer of the octopine synthase (ocs) transcriptional activating element affixed to the mannopine synthase 2' (mas2') transcriptional activating element plus minimal promoter. We tested a super-promoter/gusA fusion gene in stably transformed tobacco and maize plants, and in transiently transformed maize BMS protoplasts. In both tobacco and maize, super-promoter activity was much greater in roots than in leaves. In tobacco, super-promoter activity was greater in mature leaves than in young leaves, whereas in maize activity differed little among the tested aerial portions of the plant. When compared with other commonly used promoters (CaMV 35S, mas2', and maize ubiquitin), super-promoter activity was approximately equivalent to those of the other promoters in both maize BMS suspension cells and in stably transformed plants. The addition of a maize ubiquitin intron downstream of the super-promoter did not enhance activity in stably transformed maize.




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