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Published on September 28, 2007; 10.1104/pp.107.107599

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Received August 20, 2007
Accepted September 17, 2007

Genotypic Difference in Si Uptake and Expression of Si Transporter Genes in Rice

Jian Feng Ma *, Naoki Yamaji , Kazunori Tamai , and Namiki Mitani

Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan

* Corresponding author; email: maj{at}rib.okayama-u.ac.jp.

Rice (Oryza sativa L.) is a highly Si-accumulating species that shows genotypic differences in Si accumulation. We investigated the physiological and molecular mechanisms involved in the genotypic difference in Si uptake between the japonica variety Nipponbare and the indica variety Kasalath. Both the Si concentration in the shoot and the Si uptake per root dry weight were higher in Nipponbare than in Kasalath grown in either soil or nutrient solution. The Si uptake by a single root was also higher in Nipponbare than in Kasalath. A kinetics study showed that Nipponbare and Kasalath had a similar Km value, whereas the Vmax was higher in Nipponbare. The expression of two Si transporter genes (Lsi1 and Lsi2) investigated using real-time RT-PCR revealed higher expression of both genes in Nipponbare than in Kasalath. Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots. These results revealed that the genotypic difference in the Si accumulation results from the difference in abundance of Si transporters in rice roots.




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