Plant Physiology Preview
Published on October 5, 2007; 10.1104/pp.107.107805
OPEN ACCESS ARTICLE
Received August 21, 2007
Accepted September 26, 2007
Role of the PsbI Protein in Photosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803
Marika Dobakova , Martin Tichy , and Josef Komenda *
Institute of Microbiology, Academy of Sciences, Opatovicky mlyn, 37981 Trebon and Institute of Physical Biology, University of South Bohemia, Zamek 136, 37333 Nove Hrady, Czech Republic
* Corresponding author; email: komenda{at}alga.cz.
The involvement of the PsbI protein in the assembly and repair of the Photosystem II (PSII) complex has been studied in the cyanobacterium Synechocystis sp. PCC 6803. Analysis of PSII complexes in the wild-type strain showed that the PsbI protein was present in dimeric and monomeric core complexes, core complexes lacking CP43 and in reaction center complexes containing D1, D2 and cytochrome b-559. In addition immunoprecipitation experiments and the use of a His-tagged derivative of PsbI have revealed the presence in the thylakoid membrane of assembly complexes containing PsbI and either the precursor or mature forms of D1. Analysis of PSII assembly in the psbI deletion mutant and in strains lacking PsbI together with other PSII subunits showed that PsbI was not required for formation of PSII reaction center complexes or core complexes although levels of unassembled D1 were reduced in its absence. However, loss of PsbI led to a dramatic destabilization of CP43 binding within monomeric and dimeric PSII core complexes. Despite the close structural relationship between D1 and PsbI in the PSII complex, PsbI turned over much slower than D1, whereas high light-induced turnover of D1 was accelerated in the absence of PsbI. Overall our results suggest that PsbI is an early assembly partner for D1 and that it plays a functional role in stabilizing the binding of CP43 in the PSII holoenzyme.
This article has been cited by other articles:
|
|
|
|
 
T. Takahashi, N. Inoue-Kashino, S.-i. Ozawa, Y. Takahashi, Y. Kashino, and K. Satoh
Photosystem II Complex in Vivo Is a Monomer
J. Biol. Chem.,
June 5, 2009;
284(23):
15598 - 15606.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Dobakova, R. Sobotka, M. Tichy, and J. Komenda
Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803
Plant Physiology,
February 1, 2009;
149(2):
1076 - 1086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schottkowski, S. Gkalympoudis, N. Tzekova, C. Stelljes, D. Schunemann, E. Ankele, and J. Nickelsen
Interaction of the Periplasmic PratA Factor and the PsbA (D1) Protein during Biogenesis of Photosystem II in Synechocystis sp. PCC 6803
J. Biol. Chem.,
January 16, 2009;
284(3):
1813 - 1819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sobotka, U. Duhring, J. Komenda, E. Peter, Z. Gardian, M. Tichy, B. Grimm, and A. Wilde
Importance of the Cyanobacterial Gun4 Protein for Chlorophyll Metabolism and Assembly of Photosynthetic Complexes
J. Biol. Chem.,
September 19, 2008;
283(38):
25794 - 25802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Komenda, J. Nickelsen, M. Tichy, O. Prasil, L. A. Eichacker, and P. J. Nixon
The Cyanobacterial Homologue of HCF136/YCF48 Is a Component of an Early Photosystem II Assembly Complex and Is Important for Both the Efficient Assembly and Repair of Photosystem II in Synechocystis sp. PCC 6803
J. Biol. Chem.,
August 15, 2008;
283(33):
22390 - 22399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
