Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 19, 2007; 10.1104/pp.107.107912

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
145/4/1647    most recent
pp.107.107912v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, L.
Right arrow Articles by Wu, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, L.
Right arrow Articles by Wu, P.
Agricola
Right arrow Articles by Cheng, L.
Right arrow Articles by Wu, P.

Received August 22, 2007
Accepted October 15, 2007

Mutation in nicotianamine aminotransferease stimulated the Fe(II) acquisition system and led to iron accumulation in rice

Longjun Cheng , Fang Wang , Fangliang Huang , Luqing Zheng , Fei He , Jinhui Li , Fang-Jie Zhao , Daisei Ueno , Jian Feng Ma , Huixia Shou , and Ping Wu *

The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Zi Jin Gang campus, Hangzhou, 310058, China; Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom; Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan

* Corresponding author; email: clspwu{at}zju.edu.cn.

Higher plants acquire iron (Fe) from the rhizosphere through two strategies. Strategy II, employed by graminaceous plants, involves secretion of phytosiderophores (e.g. deoxymugineic acid in rice) by roots to solubilize Fe(III) in soil. In addition to taking up iron in the form of Fe(III)-phytosiderophore, rice also possesses the Strategy I-like system that may absorb Fe (II) directly. Through mutant screening, we isolated a rice mutant that could not grow with Fe(III)-citrate as the sole iron source, but was able to grow when Fe(II)-EDTA was supplied. Surprisingly, the mutant accumulated more iron and other divalent metals in roots and shoots than the wild-type, when both were supplied with EDTA-Fe(II) or grown under waterlogged field conditions. Furthermore, the mutant had a significantly higher concentration of iron in both unpolished and polished grains than the wild-type. Using map-based cloning method, we identified a point mutation in a gene encoding nicotianamine aminotransferease, NAAT1, which was responsible for the mutant phenotype. Because of the loss of function of NAAT1, the mutant failed to produce deoxymugineic acid and could not absorb Fe(III) efficiently. In contrast, nicotianamine (NA), the substrate for NAAT1, accumulated markedly in roots and shoots of the mutant. Microarray analysis showed that the expression of a number of the genes involved in Fe(II) acquisition was greatly stimulated in the naat1 mutant. Our results demonstrate that disruption of deoxymugineic acid biosynthesis can stimulate Fe(II) acquisition and increase iron accumulation in rice.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Dreyfus, D. Lemaire, S. Mari, D. Pignol, and P. Arnoux
Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in archaea
PNAS, September 22, 2009; 106(38): 16180 - 16184.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Zheng, F. Huang, R. Narsai, J. Wu, E. Giraud, F. He, L. Cheng, F. Wang, P. Wu, J. Whelan, et al.
Physiological and Transcriptome Analysis of Iron and Phosphorus Interaction in Rice Seedlings
Plant Physiology, September 1, 2009; 151(1): 262 - 274.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Klatte, M. Schuler, M. Wirtz, C. Fink-Straube, R. Hell, and P. Bauer
The Analysis of Arabidopsis Nicotianamine Synthase Mutants Reveals Functions for Nicotianamine in Seed Iron Loading and Iron Deficiency Responses
Plant Physiology, May 1, 2009; 150(1): 257 - 271.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Inoue, T. Kobayashi, T. Nozoye, M. Takahashi, Y. Kakei, K. Suzuki, M. Nakazono, H. Nakanishi, S. Mori, and N. K. Nishizawa
Rice OsYSL15 Is an Iron-regulated Iron(III)-Deoxymugineic Acid Transporter Expressed in the Roots and Is Essential for Iron Uptake in Early Growth of the Seedlings
J. Biol. Chem., February 6, 2009; 284(6): 3470 - 3479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists