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Published on October 19, 2007; 10.1104/pp.107.108076

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Received August 24, 2007
Accepted October 5, 2007

Two DEAD-box proteins may be part of RNA-dependent high molecular weight protein complexes in Arabidopsis thaliana mitochondria

Annemarie Matthes , Stephanie Schmidt-Gattung , Daniela Kohler , Joachim Forner , Steffen Wildum , Monika Raabe , Henning Urlaub , and Stefan Binder *

Molekulare Botanik, Universitat Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany; Max-Planck-Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry Group, Am Fassberg 11, D-37077 Gottingen, Germany

* Corresponding author; email: stefan.binder{at}uni-ulm.de.

Posttranscriptional processes are important for regulation of gene expression in plant mitochondria. DEAD-box proteins, which form a huge protein family with members from all kingdoms, are fundamental components in virtually all types of processes in the RNA metabolism. Two members of this protein family designated PMH1 and PMH2 (for putative mitochondrial RNA helicase) were analyzed and characterized in mitochondria of Arabidopsis thaliana. GFP tagging with N-terminal PMH1 and 2 sequences supports the mitochondrial localization of these proteins. Northern experiments as well as histochemical glucuronidase (GUS) staining of transgenic plants carrying respective promoter:GUS fusion constructs revealed differing transcription patterns for the two genes. In response to cold, however, transcript levels of both genes increased. Immunodetection analyses of mitochondrial protein complexes after two-dimensional blue native/urea sodium dodecyl sulfate polyacrylamide gel electrophoresis and after fractionation on sucrose gradients strongly suggest that one or both proteins are part of RNA-dependent complexes. Cold treatment of cell cultures or solubilization of mitochondria in the presence of MgCl2 favored the detection of high molecular weight complexes. This study paves the way for a detailed analysis of high molecular weight complexes in mitochondria of higher plants.




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S. Stonebloom, T. Burch-Smith, I. Kim, D. Meinke, M. Mindrinos, and P. Zambryski
Loss of the plant DEAD-box protein ISE1 leads to defective mitochondria and increased cell-to-cell transport via plasmodesmata
PNAS, October 6, 2009; 106(40): 17229 - 17234.
[Abstract] [Full Text] [PDF]


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