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Published on September 28, 2007; 10.1104/pp.107.108217

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Received August 29, 2007
Accepted September 17, 2007

A universal expression/silencing vector in plants

Yuval Peretz , Rita Mozes-Koch , Fuad Akad , Edna Tanne , Henryk Czosnek , and Ilan Sela *

The Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, Institute for Plant Sciences and Genetics, Rehovot 76100, Israel

* Corresponding author; email: sela{at}agri.huji.ac.il.

A universal vector (IL-60 and auxiliary constructs), expressing or silencing genes in every plant tested to date, is described. Plants that have been successfully manipulated by the IL-60 system include hard-to-manipulate species such as wheat, pepper, grapevine, citrus and olive. Expression or silencing develops within a few days in tomato, wheat, and most herbaceous plants and in up to 3 weeks in woody trees. Expression, as tested in tomato, is durable and persists throughout the life span of the plant. The vector is, in fact, a disarmed form of Tomato yellow leaf curl virus (TYLCV), which is applied as a double-stranded DNA and replicates as such. However, the disarmed virus does not support rolling-circle replication, and therefore viral progeny single-stranded DNA is not produced. IL-60 does not integrate into the plant's genome, and the construct, including the expressed gene, is not heritable. IL-60 is not transmitted by the TYLCV's natural insect vector. In addition, artificial satellites were constructed which require a helper virus for replication, movement and expression. With IL-60 as the disarmed helper "virus", transactivation occurs, resulting in an inducible expressing/silencing system. The system's potential is demonstrated by IL-60-derived suppression of a viral silencing suppressor of Grapevine virus A (GVA), resulting in GVA-resistant/tolerant plants.




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