Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 30, 2007; 10.1104/pp.107.108548

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
146/2/646    most recent
pp.107.108548v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, Y.
Right arrow Articles by Poirier, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, Y.
Right arrow Articles by Poirier, Y.
Agricola
Right arrow Articles by Wang, Y.
Right arrow Articles by Poirier, Y.

Received August 31, 2007
Accepted November 18, 2007

Characterization of the PHO1 gene family and the responses to phosphate deficiency of Physcomitrella patens

Yong Wang , David Secco , and Yves Poirier *

Departement de Biologie Moleculaire Vegetale, Biophore, Universite de Lausanne, CH-1015 Lausanne, Switzerland

* Corresponding author; email: yves.poirier{at}unil.ch.

PHO1 was previously identified in Arabidopsis thaliana as a protein involved in loading inorganic phosphate (Pi) into the xylem of roots and its expression was associated with the vascular cylinder. Seven genes homologous to AtPHO1 (PpPHO1;1 to PpPHO1;7) have been identified in the moss Physcomitrella patens. The corresponding proteins harbor a SPX tripartite domain in the N-terminal hydrophilic portion and an EXS domain in the conserved C-terminal hydrophobic portion, both common features of the plant PHO1 family. Northern blot analysis showed distinct expression patterns for the PpPHO1 genes, both at the tissue level and in response to phosphate deficiency. Transgenic P. patens expressing the GUS reporter gene under three different PpPHO1 promoters revealed distinct expression profiles in various tissues. Expression of PpPHO1;1 and PpPHO1;7 was specifically induced by Pi starvation. P. patens homologues to the A. thaliana PHT1, DGD2, SQD1 and APS1 genes also responded to Pi deficiency by increased mRNA levels. Morphological changes associated with Pi deficiency included elongation of caulonemata with inhibition of the formation of side-branches, resulting in colonies with greater diameter but reduced mass compared to Pi-sufficient plants. Under Pi-deficient conditions, P. patens also increased the synthesis of ribonucleases and of an acid phosphatase, and increased the ratio of sulfolipids over phospholipids. These results indicate that P. patens and higher plants share some common strategies to adapt to Pi deficiency, although morphological changes are distinct, and that the PHO1 proteins are well conserved in bryophyte despite the lack of a developed vascular system.




This article has been cited by other articles:


Home page
 J Exp BotHome page
W.-Y. Lin, S.-I Lin, and T.-J. Chiou
Molecular regulators of phosphate homeostasis in plants
J. Exp. Bot., April 1, 2009; 60(5): 1427 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. Schuette, A. J. Wood, M. Geisler, J. Geisler-Lee, R. Ligrone, and K. S. Renzaglia
Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family
Ann. Bot., March 1, 2009; 103(5): 749 - 756.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists