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Published on October 26, 2007; 10.1104/pp.107.108704

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Received September 6, 2007
Accepted October 22, 2007

A Role for F-Actin in Hexokinase Mediated Glucose Signaling

Rajagopal Balasubramanian , Abhijit Karve , Muthugapatti Kandasamy , Richard B. Meagher , and Brandon d. Moore *

Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29631; and, Genetics Department, University of Georgia, Athens, GA 30602
Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29631; Genetics Department, University of Georgia, Athens, GA 30602

* Corresponding author; email: moore8{at}clemson.edu.

Hexokinase1 (HXK1) from Arabidopsis (Arabidopsis thalian) has dual roles in glucose signaling and in glucose phosphorylation. The cellular context though for HXK1 function in either process is not well understood. Here we have shown that within normal experimental detection limits, AtHXK1 is localized continuously to mitochondria. Two mitochondrial porin proteins were identified as capable of binding to overexpressed HXK1 protein, both in vivo and in vitro. We also found that AtHXK1 can be associated with its structural homolog, F-actin, based on their co-immunoprecipitation from transgenic plants that overexpress HXK1-FLAG or from transient expression assays, and based on their localization in leaf cells after cryofixation. This association might be functionally important since glucose signaling in protoplast transient expression assays is compromised by disruption of F-actin. We also demonstrate that glucose treatment of Arabidopsis seedlings rapidly and reversibly disrupts fine mesh actin filaments. The possible roles of actin in HXK-dependent glucose signaling are discussed.




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