Plant Physiology Preview
Published on October 26, 2007; 10.1104/pp.107.108985
OPEN ACCESS ARTICLE
Received September 12, 2007
Accepted October 23, 2007
Newly Formed Vacuoles in Root Meristems of Barley and Pea Seedlings Have Characteristics of both Protein Storage and Lytic Vacuoles
Andrea Olbrich , Stefan Hillmer , Giselbert Hinz , Peter Oliviusson , and David G. Robinson *
Dept. Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
* Corresponding author; email: david.robinson{at}urz.uni-heidelberg.de.
Plant cells are considered to possess functionally different types of vacuole in the same cell. One of the papers cited in support of this concept is that of Paris et al. (2001 Cell 85: 563-572) who reported that protein storage and lytic vacuoles in root tips of barley and pea seedlings were initially separate compartments which later fused to form a central vacuole during cell elongation. We have reinvestigated the situation in these two roots using immunogold electron microscopy as well as immunofluorescence microscopy of histological sections. Using antisera generated against the whole protein of  -TIP as well as specific C-terminal TIP peptide antisera against -,  -, and  -TIP, together with antisera against the storage proteins barley lectin, and pea legumin and vicilin, we were unable to obtain evidence for separate vacuole populations. Instead, our observations point to the formation of a single type of vacuole in cells differentiating both proximally and distally from the root meristem. This is a hybrid type vacuole containing storage proteins and having both - and - TIPs, but not -TIP, in its tonoplast. As cells differentiate towards the zone of elongation their vacuoles are characterized by increasing amounts of -TIP, and decreasing amounts of -TIP.
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