Plant Physiol. Drug Metab Dispos
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Published on February 20, 2008; 10.1104/pp.107.109363

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Received September 18, 2007
Accepted February 1, 2008

Elongation changes of exploratory and root hair systems induced by ACC and AVG affect nitrate uptake and the BnNrt2.1 and BnNrt1.1 transporters genes expression in Brassica napus

Antonin Leblanc , Hugues Renault , Julien Lecourt , Philippe Etienne , Carole Deleu , and Erwan Le Deunff *

INRA, UMR 950, F-14000 Caen, France; Universite de Caen Basse-Normandie, UMR 950, Laboratoire d'Ecophysiologie Vegetale, Agronomie & Nutritions N,C,S, F-14000 Caen, France; INRA, UMR 118, F-35000 Rennes, France; Universite de Rennes, Agrocampus, UMR 118, Laboratoire d'Amelioration des Plantes et Biotechnologies Vegetales, F-35000 Rennes, France

* Corresponding author; email: erwan.ledeunff{at}unicaen.fr.

Ethylene is a plant hormone that plays a major role in the elongation of both exploratory and root hair systems. Here, we demonstrate in Brassica napus seedlings that treatments with the ethylene precursor: aminocyclopropane carboxylic acid (ACC) and the ethylene biosynthesis inhibitor: aminoethoxyvinylglycine (AVG) cause modification of the dynamic processes of primary root and root hair elongation in a dose-dependent way. Moreover, restoration of root elongation in AVG treated seedlings by 1mM L-glutamate suggested that high concentrations of AVG affect root elongation through non-overlapping ethylene metabolic pathway involving pyridoxal 5'-phosphate (PLP)-dependent enzymes of N metabolism. In this respect, treatments with high concentrations of ACC and AVG (10 µM) over five days revealed significant differences in relationships between root growth architecture and nitrate uptake capacities. Indeed, if these treatments decreased severely elongation of exploratory root system (primary root and lateral roots) they had opposing effects on root hair system. Although ACC increased the length and number of root hairs, the rate of N uptake and the transcript level of the nitrate transporter BnNrt2.1 were markedly reduced. In contrast, the decrease in root hairs length and number in AVG treated seedlings was over-compensated by an increase of nitrate uptake and BnNrt2.1 gene expression. These root architectural changes demonstrated that BnNrt2.1 expression levels were more correlated to the changes of exploratory root system than the changes of root hair system. The difference between treatments in nitrate transporters BnNrt1.1 and BnNrt2.1 genes expression is discussed with regard to presumed transport functions of BnNrt1.1 in relation to root elongation.






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