Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 9, 2008; 10.1104/pp.107.109371

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
146/3/1128    most recent
pp.107.109371v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoyerova, K.
Right arrow Articles by Zazimalova, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoyerova, K.
Right arrow Articles by Zazimalova, E.
Agricola
Right arrow Articles by Hoyerova, K.
Right arrow Articles by Zazimalova, E.

Received September 18, 2007
Accepted December 21, 2007

Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems

Klara Hoyerova , Lucie Perry *, Paul Hand , Martina Lankova , Tomas Kocabek , Sean May , Jana Kottova , Jan Paces , Richard Napier , and Eva Zazimalova

Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, Rozvojova 263, CZ-165 02 Prague 6, Czech Republic; HRI/ Univ. of Warwick, Wellesbourne, Warwick CV35 9EF, UK; Biological Centre of the Academy of Sciences of the Czech Republic, v.v.i., Institute of Plant Molecular Biology, Branisovska 31, CZ-370 05 Ceske Budejovice, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, CZ-128 00 Prague 2, Czech Republic; Institute of Molecular Genetics, the Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220, Prague 4, Czech Republic; Department of Plant Physiology, Faculty of Science, Charles University, Vinicna 5, CZ-128 44 Prague 2, Czech Republic

* Corresponding author; email: perry{at}ueb.cas.cz.

We have isolated the cDNA of the gene PaLAX1 from a wild cherry tree (Prunus avium). The gene and its product are highly similar in sequences to both the cDNAs and the corresponding protein products of AUX/LAX-type genes, coding for putative auxin influx carriers. We have prepared and characterized transformed tobacco (Nicotiana tabacum) and Arabidopsis (Arabidopsis thaliana) plants carrying the gene PaLAX1.

We have proved that constitutive overexpression of PaLAX1 is accompanied by changes in the content and distribution of free indole-3-acetic acid, the major endogenous auxin. The increase in free indole-3-acetic acid content in transgenic plants resulted in various phenotype changes, typical for the ‘auxin-overproducing’ plants. The uptake of synthetic auxin, 2,4-dichlorophenoxyacetic acid, was three times higher in transgenic lines compared to the wild type lines and the treatment with the auxin uptake inhibitor, 1-naphthoxyacetic acid, reverted the changes caused by the expression of PaLAX1. Moreover, the agravitropic response could be restored by expression of PaLAX1 in the mutant aux1 plants, which are deficient in auxin influx carrier activity.

Based on our data, we have concluded that the product of the gene PaLAX1 promotes the uptake of auxin into cells and, as a putative auxin influx carrier, it affects the content and distribution of free endogenous auxin in transgenic plants.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists