Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 15, 2008; 10.1104/pp.107.111781

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
146/4/1738    most recent
pp.107.111781v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sreenivasulu, N.
Right arrow Articles by Wobus, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sreenivasulu, N.
Right arrow Articles by Wobus, U.
Agricola
Right arrow Articles by Sreenivasulu, N.
Right arrow Articles by Wobus, U.

Received October 26, 2007
Accepted February 7, 2008

Barley grain maturation and germination: Metabolic pathway and regulatory network commonalities and differences highlighted by new MapMan/PageMan profiling tools

Nese Sreenivasulu *, Bjorn Usadel , Andreas Winter , Volodymyr Radchuk , Uwe Scholz , Nils Stein , Winfriede Weschke , Marc Strickert , Timothy J. Close , Mark Stitt , Andreas Graner , and Ulrich Wobus

Leibniz-Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3, D-06466 Gatersleben; Max-Planck-Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, D-14476 Potsdam – Golm; Department of Botany and Plant Sciences, University of California, Riverside, California 92521

* Corresponding author; email: srinivas{at}ipk-gatersleben.de.

Plant seeds prepare for germination already during seed maturation. We performed a detailed transcriptome analysis of barley grain maturation, desiccation and germination in two tissue fractions (starchy endosperm/aleurone and embryo/scutellum) using the Affymetrix barley1 chip. To aid data evaluation, Arabidopsis MapMan and PageMan tools were adapted to barley. The analyses allow a number of conclusions: (i) Cluster analysis revealed a smooth transition in transcription programs between late seed maturation and germination within embryo tissues, but not in the endosperm/aleurone. (ii) More than 12,000 transcripts are stored in the embryo of dry barley grains, many of which are presumably activated during germination. (iii) Transcriptional activation of storage reserve mobilization events occurs at an early stage of germination, well before radicle protrusion. (iv) Key genes of GA biosynthesis are already active during grain maturation at a time when ABA peaks suggesting the formation of an endogenous store of GA in the aleurone. This GA probably acts later during germination in addition to newly synthesized GA. (v) Beside the well known role of GA in gene activation during germination spatio-temporal expression data and cis-element searches in homologous rice promoters confirm an equally important gene-activating role of ABA during this developmental period. The respective regulatory webs are linked to auxin and ethylene controlled networks. In summary, new bioinformatics PageMan and MapMan tools developed in barley have been successfully used to investigate in detail the transcriptome relationships between seed maturation and germination in an important crop plant.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Thiel, D. Weier, N. Sreenivasulu, M. Strickert, N. Weichert, M. Melzer, T. Czauderna, U. Wobus, H. Weber, and W. Weschke
Different Hormonal Regulation of Cellular Differentiation and Function in Nucellar Projection and Endosperm Transfer Cells: A Microdissection-Based Transcriptome Study of Young Barley Grains
Plant Physiology, November 1, 2008; 148(3): 1436 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. T. Olsen, H. H. Divon, R. Al, K. Fosnes, S. E. Lid, and H.-G. Opsahl-Sorteberg
The defective seed5 (des5) mutant: effects on barley seed development and HvDek1, HvCr4, and HvSal1 gene regulation
J. Exp. Bot., October 1, 2008; 59(13): 3753 - 3765.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists