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Published on January 24, 2008; 10.1104/pp.107.113175

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Received November 14, 2007
Accepted January 16, 2008

Complex signaling network in regulation of adenosine 5'phosphosulfate reductase by salt stress in Arabidopsis roots

Anna Koprivova , Kathryn Anne North , and Stanislav Kopriva *

Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, UK

* Corresponding author; email: stanislav.kopriva{at}bbsrc.ac.uk.

Sulfur containing compounds play an important role in plant stress defense, however, only a little is known about the molecular mechanisms of regulation of sulfate assimilation by stress. Using known Arabidopsis mutants in signaling pathways we analyzed regulation of the key enzyme of sulfate assimilation, adenosine 5'phosphosulfate reductase (APR), by salt stress. APR activity and mRNA levels of all three APR isoforms increased three-fold in roots after 5 hours of treatment with 150 mM NaCl. The regulation of APR was not affected in mutants deficient in abscisic acid (ABA) synthesis and treatment of the plants with ABA did not affect the mRNA levels of APR isoforms, showing that APR is regulated by salt stress in an ABA independent manner. In mutants deficient in jasmonate, salicylate, or ethylene signaling APR mRNA levels were increased upon salt exposure similar to WT plants. Surprisingly, however, APR enzyme activity was not affected by salt in these plants. The same result was obtained in mutants affected in cytokinin and auxin signaling. Signaling via gibberelic acid on the other hand turned out to be essential for the increase in APR mRNA by salt treatment. These results demonstrate an extensive post-transcriptional regulation of plant APR and reveal that the sulfate assimilation pathway is controlled by a complex network of multiple signals on different regulatory levels.






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