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Published on January 24, 2008; 10.1104/pp.107.113829

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Received November 23, 2007
Accepted January 18, 2008

MYB72 Is Required in Early Signaling Steps of Rhizobacteria-Induced Systemic Resistance in Arabidopsis

Sjoerd Van der Ent , Bas W.M. Verhagen , Ronald Van Doorn , Daniel Bakker , Maarten G. Verlaan , Michiel J.C. Pel , Ruth G. Joosten , Marcel C.G. Proveniers , L.C. Van Loon , Jurriaan Ton , and Corne M.J. Pieterse *

Graduate School Experimental Plant Sciences, Plant-Microbe Interactions and Molecular Plant Physiology, Institute of Environmental Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands

* Corresponding author; email: C.M.J.Pieterse{at}uu.nl.

Colonization of Arabidopsis roots by non-pathogenic Pseudomonas fluorescens WCS417r bacteria triggers a jasmonate/ethylene-dependent induced systemic resistance (ISR) that is effective against a broad range of pathogens. Microarray analysis revealed that the R2R3-MYB-like transcription factor gene MYB72 is specifically activated in the roots upon colonization by WCS417r. Here we show that T-DNA knockout mutants myb72-1 and myb72-2 are incapable of mounting ISR against the pathogens Pseudomonas syringae pv. tomato, Hyaloperonospora parasitica, Alternaria brassicicola and Botrytis cinerea, indicating that MYB72 is essential to establish broad-spectrum ISR. Overexpression of MYB72 did not result in enhanced resistance against any of the pathogens tested, demonstrating that MYB72 is not sufficient for the expression of ISR. Yeast two-hybrid analysis revealed that MYB72 physically interacts in vitro with the ETHYLENE INSENSITIVE3 (EIN3)-LIKE3 transcription factor EIL3, linking MYB72 function to the ethylene response pathway. However, WCS417r activated MYB72 in ISR-deficient, ethylene-insensitive ein2-1 plants. Moreover, exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC) induced wild-type levels of resistance in myb72-1, suggesting that MYB72 acts upstream of ethylene in the ISR pathway. Collectively, this study identified the transcriptional regulator MYB72 as a novel ISR signaling component that is required in the roots during early signaling steps of rhizobacteria-mediated ISR.




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A. Koornneef, A. Leon-Reyes, T. Ritsema, A. Verhage, F. C. Den Otter, L.C. Van Loon, and C. M.J. Pieterse
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[Abstract] [Full Text] [PDF]


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