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Published on April 18, 2008; 10.1104/pp.107.114058

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Received December 12, 2007
Accepted April 17, 2008

Transcript profiling reveals new insights into the acclimation of the mesophilic fresh-water cyanobacterium Synechococcus elongatus PCC 7942 to iron starvation

Anke Nodop , Daniel Pietsch , Ralf Hocker , Anke Becker , Elfriede K. Pistorius , Karl Forchhammer , and Klaus-Peter Michel *

Lehrstuhl fur Molekulare Zellphysiologie, Universitat Bielefeld, Universitatsstr. 25, D-33615 Bielefeld, Deutschland; Lehrstuhl fur Genetik, Universitat Bielefeld, Universitatsstr. 25, D-33615 Bielefeld, Deutschland; Lehrstuhl fur Mikrobiologie und Organismische Interaktion, Universitat Tubingen, Auf der Morgenstelle 28, D-72076 Tubingen, Deutschland

* Corresponding author; email: klauspeter.michel{at}uni-bielefeld.de.

The regulatory network for acclimation of the obligate photoautotrophic fresh water cyanobacterium Synechococcus elongatus PCC 7942 to iron limitation was studied by transcript profiling with an oligonucleotide whole genome DNA microarray. Six regions on the chromosome with several iron-regulated genes each were identified. The irpAB and fut region encode putative iron uptake systems, the suf region participates in [Fe-S] cluster assembly under oxidative stress and iron limitation, the isiAB region encodes CP43' and flavodoxin, the idiCB region encodes the NuoE-like electron transport associated protein IdiC and the transcriptional activator IdiB, and the ackA/pgam region encodes an acetate kinase and a phosphoglycerate mutase. We also investigated the response of two Synechococcus elongatus PCC 7942 mutants to iron starvation. These were mutant K10, lacking IdiB but containing IdiC, and mutant MuD, representing a idiC-merodiploid mutant with a strongly reduced amount of IdiC as well as IdiB. The absence of IdiB in mutant K10 or the strongly reduced amount of IdiB in mutant MuD allowed for the identification of additional members of the iron-responsive IdiB regulon. Besides idiA and the irpAB operon also somB(1), somA(2), ftr1, ackA, pgam, and nat seem to be regulated by IdiB. In addition to the reduced amount of IdiB in MuD, the low concentration of IdiC may be responsible for a number of additional changes in the abundance of mainly photosynthesis-related transcripts as compared to wild-type and mutant K10. This fact may explain, why it has been impossible to obtain a fully segregated IdiC-free mutant, while it was possible to obtain a fully segregated IdiB-free mutant.






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