Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 1, 2008; 10.1104/pp.107.114090

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
146/4/1515    most recent
pp.107.114090v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Naponelli, V.
Right arrow Articles by Hanson, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Naponelli, V.
Right arrow Articles by Hanson, A. D.
Agricola
Right arrow Articles by Naponelli, V.
Right arrow Articles by Hanson, A. D.

Received November 27, 2007
Accepted January 21, 2008

Phylogenomic and functional analysis of pterin-4a-carbinolamine dehydratase family (COG2154) proteins in plants and microorganisms

Valeria Naponelli , Alexandre Noiriel , Michael J. Ziemak , Stephen M. Beverley , Lon-Fye Lye , Andrew M. Plume , Jose Ramon Botella , Karen Loizeau , Stephane Ravanel , Fabrice Rebeille , Valerie de Crecy-Lagard , and Andrew D. Hanson *

Horticultural Sciences and Microbiology and Cell Science Departments, University of Florida, Gainesville, Florida 32611; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110; Department of Botany, University of Queensland, Brisbane, Queensland 4072, Australia; and Laboratoire de Physiologie Cellulaire Vegetale, CNRS/CEA/INRA/Universite Joseph Fourier, CEA-Grenoble, F-38054 Grenoble Cedex 9, France

* Corresponding author; email: adha{at}ufl.edu.

Pterin-4a-carbinolamine dehydratases (PCDs) recycle oxidized pterin cofactors generated by aromatic amino acid hydroxylases (AAHs). PCDs are known biochemically only from animals and one bacterium, but PCD-like proteins (COG2154 in the COGs database) are encoded by many plant and microbial genomes. Since these genomes often encode no AAH homologs, the annotation of their COG2154 proteins as PCDs is questionable. Moreover, some COG2154 proteins lack canonical residues that are catalytically important in mammalian PCDs. Diverse COG2154 proteins of plant, fungal, protistan, and prokaryotic origin were therefore tested for PCD activity by functional complementation in Escherichia coli, and the plant proteins were localized using green fluorescent protein fusions. Higher and lower plants proved to have two COG2154 proteins, a mitochondrial one with PCD activity and a non-canonical, plastidial one without. Phylogenetic analysis indicated that the latter is unique to plants and arose from the former early in the plant lineage. All ten microbial COG2154 proteins tested had PCD activity; six of these came from genomes with no AAH, and six were non-canonical. The results suggested the motif [EDKH]-x(3)-H-[HN]-[PCS]-x(5,6)-[YWF]-x(9)-[HW]-x(8,15)-D as a signature for PCD activity. Organisms having a functional PCD but no AAH partner include angiosperms, yeast, and various prokaryotes. In these cases PCD presumably has another function. An ancillary role in molybdopterin cofactor metabolism, hypothesized from phylogenomic evidence, was supported by demonstrating significantly lowered activities of two molybdoenzymes in Arabidopsis (Arabidopsis thaliana) PCD knockout mutants. Besides this role, we propose that partnerless PCDs support the function of as yet unrecognized pterin-dependent enzymes.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists