Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 7, 2008; 10.1104/pp.107.114868

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
146/4/1687    most recent
pp.107.114868v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagae, M.
Right arrow Articles by Takahashi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagae, M.
Right arrow Articles by Takahashi, Y.
Agricola
Right arrow Articles by Nagae, M.
Right arrow Articles by Takahashi, Y.

Received December 11, 2007
Accepted February 2, 2008

Identification of negative cis-acting elements in response to copper in the chloroplastic iron superoxide dismutase gene of the moss Barbula unguiculata

Miwa Nagae , Masaru Nakata , and Yohsuke Takahashi *

Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan

* Corresponding author; email: ytakahas{at}hiroshima-u.ac.jp.

Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, Cu/ZnSOD and FeSOD, encoded by nuclear genes. Since bryophytes are considered as the earliest land plants, they are one of the most interesting plant models for adaptation against oxidative stress. In a previous study, we found that the FeSOD gene was expressed under copper-deficient conditions and repressed under high-copper-supply conditions; on the other hand, the Cu/ZnSOD gene was induced by copper in a moss, Barbula unguiculata. The expression of Cu/ZnSOD and FeSOD is coordinately regulated at the transcriptional level depending on metal bioavailability. Here, using transgenic moss plants, we determined that the GTACT motif is a negative cis-acting element of the moss FeSOD gene in response to copper. Furthermore, we found that a plant-specific transcription factor, PpSBP2 (for SQUAMOSA promoter-binding protein), and its related proteins bound to the GTACT motif repressed the expression of the FeSOD gene. The moss FeSOD gene was negatively regulated by copper in transgenic tobacco plants, and the Arabidopsis (Arabidopsis thaliana) FeSOD gene promoter containing the GTACT motif was repressed by copper. Our results suggested that molecular mechanisms of GTACT motif-dependent transcriptional suppression by copper are conserved in land plants.




This article has been cited by other articles:


Home page
 Plant CellHome page
H. Yamasaki, M. Hayashi, M. Fukazawa, Y. Kobayashi, and T. Shikanai
SQUAMOSA Promoter Binding Protein-Like7 Is a Central Regulator for Copper Homeostasis in Arabidopsis
PLANT CELL, January 1, 2009; 21(1): 347 - 361.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists