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Published on March 19, 2008; 10.1104/pp.107.115246

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Received December 19, 2007
Accepted March 13, 2008

Global Characterization of Cell-specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

Changqing Zhang , Georgina M. Lambert , Roger A. Barthelson , and David W. Galbraith *

Department of Plant Sciences, and BIO5 Institute for Collaborative Bioresearch, The University of Arizona, Tucson AZ 85721

* Corresponding author; email: galbraith{at}arizona.edu.

We describe a simple and highly-effective means for global identification of genes that are expressed within specific cell-types within complex tissues. It involves transgenic expression of nuclear-targeted GFP in a cell-type specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of twelve novel genes that are selectively expressed in phloem.




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