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Published on February 20, 2008; 10.1104/pp.107.115519

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Received December 26, 2007
Accepted February 12, 2008

Genetic dissection of hormonal responses in the roots of Arabidopsis thaliana grown under continuous mechanical impedance

Takashi Okamoto , Seiji Tsurumi , Kyohei Shibasaki , Yoshimi Obana , Hironori Takaji , Yutaka Oono , and Abidur Rahman *

Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, Iwate, 020-8550, Japan; Center for Supports to Research and Education Activities Isotope Division, Kobe University, Nada, Kobe, 657-8501, Japan; Graduate School of Science and Technology, Kobe University, Nada, Kobe, 657-8501, Japan; Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki, Gunma, 370-1292, Japan

* Corresponding author; email: abidur{at}iwate-u.ac.jp.

We investigated the role of ethylene and auxin in regulating the growth and morphology of roots during mechanical impedance by developing a new growing system and using the model plant Arabidopsis thaliana. The Arabidopsis seedlings grown horizontally on a dialysis membrane-covered agar plate encountered adequate mechanical impedance as the roots showed characteristic ethylene phenotypes; a two fold reduction in root growth, increase in root diameter, decrease in cell elongation and ectopic root hair formation. The root phenotype characterization of various mutants having altered response to ethylene biosynthesis or signaling, the effect of ethylene inhibitors on mechanically impeded roots and transcription profiling of the ethylene responsive genes led us to conclude that enhanced ethylene response plays a primary role in changing root morphology and development during mechanical impedance. Further, the differential sensitivity of horizontally and vertically grown roots toward exogenous ethylene suggested that ethylene signaling plays a critical role in enhancing the ethylene response. We subsequently demonstrated that the enhanced ethylene response also affects the auxin response in root. Taken together, our results provide a new insight into the role of ethylene in changing root morphology during mechanical impedance.






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