Plant Physiol. Drug Metab Dispos
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Published on April 23, 2008; 10.1104/pp.107.115584

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Received January 10, 2008
Accepted April 15, 2008

Photosynthetic utilization of HCO3- in Zostera marina is reduced by inhibitors of mitochondrial ATPase and electron transport

Herman Carr * and Lennart Axelsson

Department of Botany, Stockholm University, SE-106 91 Stockholm, Sweden; Kristineberg Marine Research Station, SE-450 34 Fiskebackskil, Sweden

* Corresponding author; email: carr{at}botan.su.se.

When Zostera marina (Linnaeus) was irradiated after a period of darkness, initiation of photosynthetic O2 evolution occurred in two phases. During a lag phase, lasting 4-5 minutes, photosynthesis was supported by a diffusive entry of CO2. Photosynthesis then rapidly increased to its full rate. Tris buffer, at a concentration of 50 mM, completely inhibited this increase without affecting CO2-supported photosynthesis during the lag phase. These results verify that the increase in photosynthesis after the lag phase depended on an activation of HCO3- utilization through acid zones generated by proton pumps located to the outer cell membrane. In similar experiments, 6.25 µM of the mitochondrial ATPase blocker oligomycin inhibited photosynthetic HCO3- utilization by more than 60 %. Antimycin A, a selective blocker of mitochondrial electron transport, caused a similar inhibition of HCO3- utilization. Measurements at elevated CO2 concentrations verified that neither oligomycin nor antimycin interfered with linear photosynthetic electron transport or with CO2 fixation. Thus, a major part of the ATP used for the generation of acid zones involved in HCO3- utilization in Zostera marina was derived from mitochondrial respiration.






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