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Published on February 27, 2008; 10.1104/pp.107.115592

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Received January 20, 2008
Accepted January 31, 2008

Global Transcript Levels respond to Small Changes of the Carbon Status during a Progressive Exhaustion of Carbohydrates in Arabidopsis Rosettes

Bjorn Usadel *, Oliver E. Blasing , Yves Gibon , Kristin Retzlaff , Melanie Hohne , Manuela Gunther , and Mark Stitt

Max Planck Institute of Molecular Plant Physiology, Science Park Golm, Am Muehlenberg 1, 14476 Potsdam-Golm

* Corresponding author; email: usadel{at}mpimp-golm.mpg.de.

The balance between the supply and utilization of carbon changes continually. Smith and Stitt (2007) proposed that plants respond in an ‘acclimatory’ manner, modifying carbon utilization to minimise harmful periods of carbon depletion. This hypothesis predicts that signalling events are initiated by small changes in the carbon status. We analysed the global transcriptional response to a gradual depletion of carbon during the night and an extension of the night, where carbon becomes severely limiting from four hours onwards. The response was interpreted using published datasets for sugar, light and circadian responses, Hundreds of carbon-responsive genes respond during the night and others very early in the extended night. Pathway analysis reveals that biosynthesis and cellular growth genes are repressed during the night, and genes involved in catabolism are induced during the first hours of the extended night. The carbon-response is amplified by an antagonistic interaction with the clock. Light-signaling is attenuated during the 24 hour light/dark cycle. A model was developed, which uses the response of 22K genes during a circadian cycle and their responses to carbon and light to predict global transcriptional responses during diurnal cycles of wild-type and starchless pgm mutant plants and an extended night in wild-type plants. By identifying sets of genes that respond at different speeds and times during C-depletion, our extended data set and model aids the analysis of candidates for C-signalling. This is illustrated for AKIN10 and four bZIP transcription factors, and sets of genes involved in trehalose signalling, protein turnover and starch breakdown.




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