Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 27, 2008; 10.1104/pp.108.115832

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
146/4/1663    most recent
pp.108.115832v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thakare, D.
Right arrow Articles by Perry, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thakare, D.
Right arrow Articles by Perry, S. E.
Agricola
Right arrow Articles by Thakare, D.
Right arrow Articles by Perry, S. E.

Received January 3, 2008
Accepted February 21, 2008

The MADS-domain transcriptional regulator AGAMOUS-Like 15 promotes somatic embryo development in Arabidopsis thaliana and Glycine max

Dhiraj Thakare , Weining Tang , Kristine Hill , and Sharyn E. Perry *

Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312; Center for Medical Genomics, Emory University School of Medicine, Atlanta, GA 30033-5307; Dartmouth College, Department of Biological Sciences, Hanover, NH 03755

* Corresponding author; email: sperr2{at}email.uky.edu.

The MADS-domain transcriptional regulator AGL15 has been reported to enhance somatic embryo development when constitutively expressed. Here we report that loss-of-function mutants of AGL15, alone or when combined with a loss-of-function mutant of a closely related family member, AGL18, show decreased ability to produce somatic embryos. If constitutive expression of orthologs of AGL15 is able to enhance somatic embryo development in other species, thereby facilitating recovery of transgenic plants, then AGL15 may provide a valuable tool for crop improvement. To test this idea in Glycine max, a full-length cDNA encoding a putative ortholog of AGL15 was isolated from soybean somatic embryos. Subsequently the corresponding genomic region of the gene was obtained. This gene, designated GmAGL15, encodes a protein with highest similarity to AGL15 from Arabidopsis thaliana and Brassica napus that accumulates to its highest amount in embryos in these species. Like Arabidopsis and Brassica AGL15, GmAGL15 was preferentially expressed in developing embryos. When ectopically overexpressed the soybean protein was able to enhance somatic embryo development in soybean.




This article has been cited by other articles:


Home page
 Plant CellHome page
Y. Zheng, N. Ren, H. Wang, A. J. Stromberg, and S. E. Perry
Global Identification of Targets of the Arabidopsis MADS Domain Protein AGAMOUS-Like15
PLANT CELL, September 1, 2009; 21(9): 2563 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
H. Zhang and J. Ogas
An Epigenetic Perspective on Developmental Regulation of Seed Genes
Mol Plant, July 1, 2009; 2(4): 610 - 627.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. E. Nolan, S. Kurdyukov, and R. J. Rose
Expression of the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula
J. Exp. Bot., April 1, 2009; 60(6): 1759 - 1771.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Nakaminami, K. Hill, S. E. Perry, N. Sentoku, J. A. Long, and D. T. Karlson
Arabidopsis cold shock domain proteins: relationships to floral and silique development
J. Exp. Bot., March 1, 2009; 60(3): 1047 - 1062.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists