Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 14, 2008; 10.1104/pp.108.115956

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
147/4/1603    most recent
pp.108.115956v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Persia, D.
Right arrow Articles by Cresti, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Persia, D.
Right arrow Articles by Cresti, M.
Agricola
Right arrow Articles by Persia, D.
Right arrow Articles by Cresti, M.

Received January 7, 2008
Accepted March 9, 2008

Sucrose synthase is associated with the cell wall of tobacco pollen tubes

Diana Persia , Giampiero Cai , Cecilia Del Casino , Claudia Faleri , Michiel T.M. Willemse , and Mauro Cresti

Dipartimento Scienze Ambientali "G. Sarfatti", Universita di Siena, via Mattioli 4, 53100 Siena (Italy); Plant Cytology and Morphology, Arboretumlaan 4, 6703 BD Wageningen University, Wageningen (The Netherlands)

Sucrose synthase (EC-Number 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since sucrose synthase is important for plant cell growth, insights into its structure, localization and features is useful for defining the relationships between nutrients, growth and cell morphogenesis. We used the pollen tube of Nicotiana tabacum L. as a cell model to characterize the main features of sucrose synthase with regards to cell growth and cell wall synthesis. Apart from its role during sexual reproduction, the pollen tube is a typical tip-growing cell and the proper construction of its cell wall is essential for correct shaping and direction of growth. The outer cell wall layer of pollen tubes consists of pectins but the inner layer is composed of cellulose and callose; both polymers require metabolic precursors in the form of UDP-glucose, which is synthesised by sucrose synthase. We identified an 88-kD polypeptide in the soluble, plasma membrane and Golgi fraction of pollen tubes. The protein was also found in association with the cell wall. After purification, the protein showed an enzyme activity similar to that of maize sucrose synthase. Distribution of sucrose synthase was affected by Brefeldin A and depended on the nutrition status of the pollen tube because an absence of metabolic sugars in the growth medium caused sucrose synthase to distribute differently during tube elongation. Analysis by bidimensional electrophoresis indicated that sucrose synthase exists as two isoforms, one of which is phosphorylated and more abundant in the cytoplasm and cell wall, while the other is not phosphorylated and is specific to the plasma membrane. Results indicate that the protein has a role in construction of the extracellular matrix and thus in the morphogenesis of pollen tubes.




This article has been cited by other articles:


Home page
 Mol PlantHome page
B. Cubero, Y. Nakagawa, X.-Y. Jiang, K.-J. Miura, F. Li, K. G. Raghothama, R. A. Bressan, P. M. Hasegawa, and J. M. Pardo
The Phosphate Transporter PHT4;6 Is a Determinant of Salt Tolerance that Is Localized to the Golgi Apparatus of Arabidopsis
Mol Plant, May 1, 2009; 2(3): 535 - 552.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Y. Cheung and S. C. de Vries
Membrane Trafficking: Intracellular Highways and Country Roads
Plant Physiology, August 1, 2008; 147(4): 1451 - 1453.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists