Plant Physiology Preview
Published on June 11, 2008; 10.1104/pp.108.116632
OPEN ACCESS ARTICLE
Received January 21, 2008
Accepted May 27, 2008
Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells
Akira Endo , Yoshiaki Sawada , Hirokazu Takahashi , Masanori Okamoto , Keiichi Ikegami , Hanae Koiwai , Mitsunori Seo , Tomonobu Toyomasu , Wataru Mitsuhashi , Kazuo Shinozaki , Mikio Nakazono , Yuji Kamiya , Tomokazu Koshiba , and Eiji Nambara *
Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachiouji, Tokyo 192-0397, Japan; Growth Regulation Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan; Course of the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University, Morioka, Iwate 020-8550, Japan; Department of Bioresource Engineering, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan; Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan; Gene Discovery Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan
* Corresponding author; email: nambara{at}postman.riken.jp.
The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In the present study, we examined the tissue-specific localization of ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis (Arabidopsis thaliana (L.) Heynh.) plants by using specific antibodies against AtNCED3, AtABA2 and AAO3. Immunohistochemical analysis revealed that in turgid plants AtABA2 and AAO3 proteins were localized in vascular parenchyma cells most abundantly at the boundary between xylem and phloem bundles, but that the AtNCED3 protein was undetectable in these tissues. In water-stressed plants, AtNCED3 was detected exclusively in the vascular parenchyma cells together with AtABA2 and AAO3. In situ hybridization using the antisense probe for AtNCED3 showed that the drought-induced expression of AtNCED3 was also restricted to the vascular tissues. Expression analysis on laser microdissected cells revealed that, among 9 drought-inducible genes examined, the early induction of most genes were spatially restricted to vascular cells at 1 hour and then some spread to mesophyll cells at 3 hours. The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight in plant systemic response to drought stresses.
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