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Published on February 27, 2008; 10.1104/pp.108.117432

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Received February 4, 2008
Accepted February 20, 2008

Differential regulation of root arginine catabolism and polyamine metabolism in clubroot-susceptible and partially resistant Arabidopsis genotypes

Melanie Jubault , Celine Hamon , Antoine Gravot , Christine Lariagon , Regine Delourme , Alain Bouchereau , and Maria J. Manzanares-Dauleux *

UMR 118 INRA-Agrocampus Rennes, Amelioration des Plantes et Biotechnologies Vegetales, BP35327, 35653 Le Rheu Cedex, France; UMR 6026 CNRS-Universite de Rennes 1, Interactions Cellulaires et Moleculaires, Campus de Beaulieu, CS 74205, 35042 Rennes Cedex, France

* Corresponding author; email: maria.manzanares{at}agrocampus-rennes.fr.

The hypertrophy and hyperplasia of infected roots into clubs are the intrinsic characteristics of clubroot, one of the economically most important diseases in Brassica crops worldwide. Polyamines, arginine-derived metabolites, have long been recognized as cell proliferation and differentiation regulators in plants and are consequently suitable candidates for potential gall development factors. Furthermore, arginine catabolism, through arginase, which is strongly connected to polyamine metabolism, would play an important role in response to wound trauma and pathogen infection. In this study, we exploited the Arabidopsis thaliana-Plasmodiophora brassicae pathosystem to investigate the involvement of polyamine metabolism and arginine catabolism in host responses to the pathogen infection and in partial clubroot resistance mechanisms. We demonstrated at the transcriptional, enzymatic and metabolic levels that polyamine metabolism and arginine catabolism are induced during the later stages of disease in compatible Arabidopsis thaliana - Plasmodiophora brassicae interactions. However, susceptible and partially resistant plants showed strikingly different arginine metabolism signatures. Susceptible plants were characterized by a transient agmatine production, a massive induction of arginase and a strong accumulation of proline. The potential functions of this marked activation of the arginase pathway in the Plasmodiophora brassicae pathogenicity strategy are discussed. Partially resistant plants showed a continuous agmatine production and a weaker arginase pathway activity than the susceptible genotype. Results suggest that the symptom severity was strongly associated to the differential regulation of root polyamine metabolism and arginine catabolism. Further work using arginase transgenic plants will provide insight into the physiological function of the arginase pathway in partial clubroot resistance.




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