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Published on June 13, 2008; 10.1104/pp.108.117754

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Received February 13, 2008
Accepted June 11, 2008

Metabolome Analysis of Biosynthetic Mutants Reveals Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis thaliana

Christoph Böttcher , Edda v. Roepenack-Lahaye , Jürgen Schmidt , Constanze Schmotz , Steffen Neumann , Dierk Scheel , and Stephan Clemens *

Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle/Saale, Germany; Leibniz Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, 06120 Halle/Saale, Germany; University of Bayreuth, Department of Plant Physiology, Universitätsstrasse 30, 95449 Bayreuth, Germany

* Corresponding author; email: stephan.clemens{at}uni-bayreuth.de.

Metabolomics is facing a major challenge: the lack of knowledge about metabolites present in a given biological system. Thus, large-scale discovery of metabolites is considered an essential step towards a better understanding of plant metabolism. We show here that the application of a metabolomics approach generating structural information for the analysis of A. thaliana mutants allows efficient cataloging of metabolites. 56 % of the features that showed significant differences in abundance between seeds of wild type, tt4 and tt5 mutant plants could be annotated. Seventy five compounds were structurally characterized, 21 of which could be identified. About 40 compounds had not been known from A. thaliana before. Also, the high resolution analysis revealed an unanticipated expansion of metabolic conversions upstream of biosynthetic blocks. Deficiency in chalcone synthase results in the increased seed-specific biosynthesis of a range of phenolic choline esters. Similarly, a lack of chalcone isomerase activity leads to the accumulation of various naringenin chalcone derivatives. Furthermore, our data provide insight into the connection between p-coumaroyl-CoA-dependent pathways. Lack of flavonoid biosynthesis results in elevated synthesis not only of p-coumarate derived choline esters but also of sinapate-derived metabolites. However, sinapoylcholine is not the only accumulating end product. Instead, we observed specific and sophisticated changes in the complex pattern of sinapate-derivatives.




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