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Published on April 23, 2008; 10.1104/pp.108.118661

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Received March 5, 2008
Accepted April 4, 2008

Reduction of plastid-localized CA activity results in reduced Arabidopsis thaliana seedling survivorship

Fernando J. Ferreira , Cathy Guo , and John R. Coleman *

Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, CANADA M5S 3B2

* Corresponding author; email: Coleman{at}csb.utoronto.ca.

Carbonic anhydrase (CA; EC 4.2.1.1) catalyses the interconversion of CO2 and HCO3- and is a major protein constituent of the C3 higher plant chloroplast where it is presumed to play a role in photosynthetic carbon assimilation. In this study we have used both RNA antisense and gene knockout lines to specifically reduce the activity of the chloroplast {beta}CA1 polypeptide (At3g01500) in the model plant Arabidopsis. Although able to germinate, seedling establishment of transgenic plants is significantly reduced relative to wild type plants when grown at ambient levels of CO2. Growth at elevated (1500 µl·L-1) CO2 or on plates supplemented with sucrose restores seedling establishment rates to wild type levels. Seed from wild type and transgenic plants exhibited no significant differences in seed protein, and lipid content, or reserve mobilization during seedling growth. {beta}CA1 deficient seedlings do, however, exhibit reduced capacity for light dependent 14CO2 assimilation prior to the development of true leaves. The small number of surviving seedlings able to grow and develop are phenotypically similar to wild type plants, even when subsequently grown at sub-ambient levels of CO2. Microarray analysis of mature leaves of {beta}CA1 deficient plants show some differences in transcript abundance, particularly with genes involved in ethylene signalling and response. The data suggest that reduced levels of seedling establishment by {beta}CA1 deficient plants could be the result of poor cotyledon photosynthetic performance at the onset of phototrophic growth and prior to the development of true leaves.




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S. K. Tanz, S. G. Tetu, N. G.F. Vella, and M. Ludwig
Loss of the Transit Peptide and an Increase in Gene Expression of an Ancestral Chloroplastic Carbonic Anhydrase Were Instrumental in the Evolution of the Cytosolic C4 Carbonic Anhydrase in Flaveria
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