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Published on July 9, 2008; 10.1104/pp.108.118851

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Received March 7, 2008
Accepted July 7, 2008

Manganese efficiency in barley: identification and characterization of the metal ion transporter HvIRT1

Pai Pedas *, Cecilie K. Ytting , Anja T. Fuglsang , Thomas P. Jahn , Jan K. Schjoerring , and Soren Husted

Plant and Soil Science Laboratory, Department of Agricultural Sciences, Department of Plant Biology and Biotechnology, Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Department of Plant Biology and Biotechnology, University of Copenhagen, The Faculty of Life Sciences, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark

* Corresponding author; email: pp{at}life.ku.dk.

Manganese (Mn) deficiency is an important plant nutritional disorder in many parts of the world. Barley (Hordeum vulgare) genotypes differ considerably in their ability to grow in soils with low Mn2+ availability. Differential genotypic Mn efficiency can be attributed to differences in Mn2+ uptake kinetics in the low nM concentration range. However, the molecular basis for these differences has not yet been clarified. We here present the identification and characterization of the first barley gene encoding a plasma membrane localized metal transport protein being able to transport Mn2+. The gene is designated HvIRT1 because it belongs to the ZIP gene family and has a high similarity to OsIRT1. A novel yeast uptake assay based on ICP-MS analysis of 31 different metal and metalloid ions showed that the HvIRT1 protein, in addition to Mn2+, also transported Fe2+/Fe3+, Zn2+ and Cd2+. Both Mn and Fe deficiency induced an up-regulation of HvIRT1 in two barley genotypes differing in Mn efficiency but the expression levels were in all cases highest (up to 40%) in the Mn-efficient genotype. The higher expression of HvIRT1 correlated with an increased Mn2+ uptake rate. It is concluded that HvIRT1 is an important component controlling Mn2+ uptake in barley roots and HvIRT1 contributes to genotypic differences in Mn2+ uptake kinetics.




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S. Husted, K. H. Laursen, C. A. Hebbern, S. B. Schmidt, P. Pedas, A. Haldrup, and P. E. Jensen
Manganese Deficiency Leads to Genotype-Specific Changes in Fluorescence Induction Kinetics and State Transitions
Plant Physiology, June 1, 2009; 150(2): 825 - 833.
[Abstract] [Full Text] [PDF]


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