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Published on June 6, 2008; 10.1104/pp.108.118919

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Received March 14, 2008
Accepted May 28, 2008

The transcription factor VvMYB5b contributes to the regulation of anthocyanin and proanthocyanidin biosynthesis in developing grape berries

Laurent Deluc , Jochen Bogs , Amanda R. Walker , Thilia Ferrier , Alain Decendit , Jean-Michel Merillon , Simon P. Robinson , and Francois Barrieu *

Institut des Sciences de la Vigne et du Vin, UMR 1287 Ecophysiologie et Genomique Fonctionnelle de la Vigne - INRA, Universite de Bordeaux 1, Universite Victor Segalen Bordeaux 2, Centre INRA de Bordeaux-BP81, 33883 Villenave d'Ornon, France; Groupe d'Etude des Substances Vegetales a Activite Biologique, EA 3675, ISVV, Universite Victor Segalen Bordeaux 2, UFR Sciences Pharmaceutiques, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France; CSIRO Plant Industry, PO Box 350, Glen Osmond SA 5064, Australia

* Corresponding author; email: francois.barrieu{at}bordeaux.inra.fr.

Among the dramatic changes occurring during grape berry development, those affecting the flavonoid pathway have provoked a number of investigations in the last ten years. In addition to producing several compounds involved in the protection of the berry and the dissemination of the seeds, final products of this pathway also play a critical role in berry and wine quality. In this work, we describe the cloning and functional characterization of VvMYB5b, a cDNA isolated from a Vitis vinifera L. cv. Cabernet Sauvignon berry library. VvMYB5b encodes a protein belonging to the R2R3-MYB family of transcription factors and displays significant similarity with VvMYB5a, another MYB factor recently shown to regulate flavonoid synthesis in grapevine. The ability of VvMYB5a and VvMYB5b to activate the grapevine promoters of several structural genes of the flavonoid pathway was confirmed by transient expression of the corresponding cDNAs in grape cells. Over-expression of VvMYB5b in tobacco leads to an up-regulation of genes encoding enzymes of the flavonoid pathway and results in the accumulation of anthocyanin- and proanthocyanidin-derived compounds. The ability of VvMYB5b to regulate particularly the anthocyanin and the PAs pathways is discussed in relation to other recently characterized MYB transcription factors in grapevine. Taken together, data presented in this paper are giving new insights to unravel the transcriptional mechanisms associated with the regulation of the flavonoid pathway throughout grape berry development.




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