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Published on May 28, 2008; 10.1104/pp.108.119081

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Received March 17, 2008
Accepted May 25, 2008

Sequence analysis of BAC clones from the Apospory-Specific Genomic Region (ASGR)of Pennisetum and Cenchrus

Joann A. Conner , Shailendra Goel , Gunawati Gunawan , Marie-Michele Cordonnier-Pratt , Virgil Ed Johnson , Chun Liang , Haiming Wang , Lee H. Pratt , John E. Mullet , Jeremy DeBarry , Lixing Yang , Jeffrey L. Bennetzen , Patricia E. Klein , and Peggy Ozias-Akins *

Department of Horticulture, University of Georgia, Tifton, GA 31793-0748; Department of Plant Biology, Department of Genetics, and Office of Research Services University of Georgia, Athens, GA 30602; Department of Plant Biology Institute for Plant Genomics and Biotechnology,, Texas A&M University, College Station, TX 77843

* Corresponding author; email: pozias{at}uga.edu.

Apomixis, asexual reproduction through seed, is widespread among angiosperm families. Gametophytic apomixis in Pennisetum squamulatum and Cenchrus ciliaris is controlled by the apospory-specific genomic region (ASGR), which is highly conserved and macrosyntenic between these species. Thirty-two ASGR BACs isolated from both species and one ASGR-recombining BAC from P. squamulatum, that together cover approximately 2.7 Mb of DNA, were used to investigate the genomic structure of this region. Phrap assembly of 4521 high quality reads generated 1341 contiguous sequences (contigs) (730 from the ASGR and 30 from the ASGR-recombining BAC in P. squamulatum, plus 580 from the C. ciliaris ASGR). Contigs containing putative protein-coding regions unrelated to transposable elements were identified based on protein similarity after BlastX analysis. These putative coding regions were further analyzed in silico with reference to the rice and sorghum genomes using the resources at Gramene (www.gramene.org) and Phytozome (www.phytozome.net) and by hybridization against sorghum BAC filters. The ASGR sequences reveal that the ASGR (1) contains both gene-rich and gene-poor segments, (2) contains several genes that may play a role in apomictic development, (3) has many classes of transposable elements, and (4) does not exhibit large scale synteny with either rice or sorghum genomes but does contain multiple regions of microsynteny with these species.






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