Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 28, 2008; 10.1104/pp.108.119339

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
147/3/1225    most recent
pp.108.119339v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Merigout, P.
Right arrow Articles by Daniel-Vedele, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Merigout, P.
Right arrow Articles by Daniel-Vedele, F.
Agricola
Right arrow Articles by Merigout, P.
Right arrow Articles by Daniel-Vedele, F.

Received March 18, 2008
Accepted May 16, 2008

PHYSIOLOGICAL AND TRANSCRIPTOMIC ASPECTS OF UREA UPTAKE AND ASSIMILATION IN ARABIDOPSIS PLANTS

Patricia Merigout , Maud Lelandais , Frederique Bitton , Jean-Pierre Renou , Xavier Briand , Christian Meyer , and Francoise Daniel-Vedele *

INRA, IJPB, Unite de Nutrition Azotee des Plantes, F-78000 Versailles, France; INRA, Unite Mixte de Recherche en Genomique Vegetale, F-91057 Evry, France; BiotechMarine BP 65, 22260 Pontrieux, France

* Corresponding author; email: vedele{at}versailles.inra.fr.

Urea is the major nitrogen (N) form supplied as fertilizer in agriculture but also an important N metabolite in plants. Urea transport and assimilation were investigated in Arabidopsis. Uptake studies using 15N-labelled urea demonstrated the capacity of Arabidopsis to absorb urea, and that the urea uptake was regulated by the initial N status of the plants. Urea uptake was stimulated by urea, but was reduced by the presence of ammonium nitrate in the growth medium. N deficiency in plants did not affect urea uptake. Urea exerted a repressive effect on nitrate influx whereas urea enhanced ammonium uptake. The use of [15N]urea and [15N]ammonium tracers allowed us to show that urea and ammonium assimilation pathways were similar. Finally, urea uptake was less efficient than nitrate uptake, and urea grown-plants presented signs of N starvation. We also report the first analysis of Arabidopsis gene expression profiling in response to urea. Our transcriptomic approach revealed that nitrate and ammonium transporters were transcriptionally regulated by urea, as well as key enzymes of the GS-GOGAT pathway. AtDUR3, a high-affinity urea transporter in Arabidopsis, was strongly up-regulated by urea. Moreover, our transcriptomic data suggest that other genes are also involved in urea influx.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists