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Published on July 3, 2008; 10.1104/pp.108.119461

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Received March 19, 2008
Accepted June 25, 2008

A Role for a Menthone Reductase in Resistance against Microbial Pathogens in Plants

Hyong Woo Choi , Byung Gil Lee , Nak Hyun Kim , Yong Park , Chae Woo Lim , Hyun Kyu Song , and Byung Kook Hwang *

Laboratory of Molecular Plant Pathology and Laboratory of Structural Biology, School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-713, Republic of Korea

* Corresponding author; email: bkhwang{at}korea.ac.kr.

Plants elaborate a vast array of enzymes that synthesize defensive secondary metabolites in response to pathogen attack. Here, we isolated the pathogen responsive-CaMNR1 (menthone: (+)-(3S)-neomenthol reductase) gene, a member of the short-chain dehydrogenase/reductase (SDR) superfamily, from pepper (Capsicum annuum) plants. Gas chromatography-mass spectrometry analysis revealed that purified CaMNR1 and its ortholog AtSDR1 catalyze a menthone reduction with NADPH as a cofactor to produce neomenthol with antimicrobial activity. CaMNR1 and AtSDR1 also possess a significant catalytic activity for neomenthol oxidation. We examined the cellular function of the CaMNR1 gene by virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis plants, respectively. CaMNR1-silenced pepper plants were significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and expressed lower levels of salicylic acid (SA)-responsive CaBPR1 and CaPR10 and jasmonic acid (JA)-responsive CaDEF1. CaMNR1-overexpression (OX) Arabidopsis plants exhibited enhanced resistance to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato DC3000 and the biotrophic pathogen Hyaloperonospora parasitica isolate Noco2, accompanied by induction of AtPR1 and AtPDF1.2. In contrast, mutation in the CaMNR1 ortholog Arabidopsis SDR1 (AtSDR1) significantly enhanced susceptibility to both pathogens. Together, these results indicate that the novel menthone reductase gene CaMNR1 and its ortholog AtSDR1 positively regulate plant defenses against a broad spectrum of pathogens.




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