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Published on June 26, 2008; 10.1104/pp.108.119842

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Received March 26, 2008
Accepted June 19, 2008

Synthetic Lipid (DOPG) Vesicles Accumulate in the Cell Plate Region but do Not Fuse

Agnieszka Esseling-Ozdoba , Jan W. Vos , Andre A.M. van Lammeren , and Anne Mie C. Emons *

Laboratory of Plant Cell Biology, Department of Plant Sciences, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands; FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1009 DB Amsterdam, The Netherlands

* Corresponding author; email: annemie.emons{at}wur.nl.

The cell plate is the new cell wall, with bordering plasma membrane, that is formed between two daughter cells in plants, and it is formed by fusion of vesicles (~60 nm). To start to determine physical properties of cell plate forming vesicles for their transport through the phragmoplast, and fusion with each other, we microinjected fluorescent synthetic lipid vesicles that were made of 1,2-Dioleoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (DOPG) into Tradescantia virginiana stamen hair cells. During interphase, the 60 nm wide DOPG vesicles moved inside the cytoplasm comparably to organelles. During cytokinesis, they were transported through the phragmoplast and accumulated in the cell plate region together with the endogenous vesicles, even inside the central cell plate region. Since at this stage microtubules are virtually absent from that region, while actin filaments are present, actin filaments may have a role in the transport of vesicles towards the cell plate. Unlike the endogenous vesicles, the synthetic DOPG vesicles did not fuse with the developing cell plate. Instead, they redistributed into the cytoplasm of the daughter cells upon completion of cytokinesis. Since the redistribution of the vesicles occurs when actin filaments disappear from the phragmoplast, actin filaments may be involved in keeping the vesicles inside the developing cell plate region.




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