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Published on May 28, 2008; 10.1104/pp.108.119925

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Received March 27, 2008
Accepted May 19, 2008

SCAMPs HIGHLIGHT THE DEVELOPING CELL PLATE DURING CYTOKINESIS IN TOBACCO BY-2 CELLS

Sheung Kwan Lam , Yi Cai , Stefan Hillmer , David G. Robinson , and Liwen Jiang *

Department of Biology and Molecular Biotechnology Program, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China; Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, Heidelberg, Germany

* Corresponding author; email: ljiang{at}cuhk.edu.hk.

We have previously demonstrated that OsSCAMP1-YFP in transgenic tobacco BY-2 cells locates to the plasma membrane and to motile punctate structures, which represent the TGN/early endosome and are tubule-vesicular in nature (Lam et al., 2007a). Here we now show that SCAMPs are diverted to the cell plate during cytokinesis dividing BY-2 cells. As cells progress from metaphase to cytokinesis, punctate OsSCAMP1-labeled structures begin to collect in the future division plane. Together with the internalized endosomal marker FM4-64 they then become incorporated into the cell plate as it forms and expands. This was confirmed by immunogold EM. We have also monitored for the Golgi apparatus and the prevacuolar compartment (PVC)/multivesicular body (MVB). Golgi stacks tend to accumulate in the vicinity of the division plane, but the signals are clearly separate to the cell plate. The situation with the PVC (labeled by GFP-BP-80) is not so clear. Punctate BP-80 signals are seen at the advancing periphery of the cell plate, which was confirmed by immunogold EM. Specific but weak labelling was observed in the cell plate, but no evidence for a fusion of the PVC/MVB with the cell plate could be obtained. Our data therefore supports the notion that cell plate formation is mainly a secretory process involving mass incorporation of domains of the TGN/early endosome membrane. We regard the involvement of multivesicular late endosomes in this process to be equivocal.




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