Plant Physiology Preview
Published on June 26, 2008; 10.1104/pp.108.120196
OPEN ACCESS ARTICLE
Received April 2, 2008
Accepted June 22, 2008
Genetic evidence that cellulose synthase activity influences microtubule cortical array organization
Alexander R. Paredez , Staffan Persson , David W. Ehrhardt , and Chris R. Somerville *
Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA; Department of Molecular and Cell Biology, UC Berkeley, CA 94720, USA; Department of Biological Sciences, Stanford, CA 94305, USA; Max-Planck-Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 2, 14476 Golm, Germany
* Corresponding author; email: crs{at}berkeley.edu.
To identify factors that influence cytoskeletal organization we screened for Arabidopsis thaliana mutants that show hypersensitivity to the microtubule destabilizing drug oryzalin. We cloned the genes corresponding to two of the 131 mutant lines obtained. The genes encoded mutant alleles of PROCUSTE1 and KORRIGAN, which both encode proteins that have previously been implicated in cellulose synthesis. Analysis of microtubules in the mutants revealed that both mutants have altered orientation of root cortical microtubules. Similarly, isoxaben an inhibitor of cellulose synthesis also caused altered orientation of cortical microtubules while exogenous cellulose degradation did not. Thus, our results substantiate that proteins involved in cell wall biosynthesis influence cytoskeletal organization and indicate that this influence on cortical microtubule stability and orientation is correlated with cellulose synthesis rather than the integrity of the cell wall.
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