Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on June 6, 2008; 10.1104/pp.108.120402

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Received April 2, 2008
Accepted June 2, 2008

A comparison of leaf and petal senescence in wallflowers (Erysimum linifolium) reveals common and distinct patterns of gene expression and physiology

A.M. Price , D. F. Aros Orellana , F. Mohd Salleh , R. Stevens , R. Acock , V. Buchanan-Wollaston , A. D. Stead , and H.J. Rogers *

Cardiff School of Biosciences, Main Building, Park Place, Cardiff, CF10 3TL, UK; Warwick HRI, University of Warwick, Wellesbourne, Warwick, Warwickshire, CV35 9EF, UK; School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

* Corresponding author; email: rogershj{at}cf.ac.uk.

Petals and leaves share common evolutionary origins, but perform very different functions. However, few studies have compared leaf and petal senescence within the same species. Wallflowers (Erisymum linifolium), an ornamental species closely related to Arabidopsis, provide a good species in which to study these processes. Physiological parameters were used to define stages of development and senescence in leaves and petals and to align these stages in the two organs. Treatment with silver thiosulphate confirmed that petal senescence in wallflowers is ethylene dependent, and treatment with exogenous cytokinin and 6-methyl purine, an inhibitor of cytokinin oxidase, suggests a role for cytokinins in this process. Subtractive libraries were created, enriched for wallflower genes whose expression is up-regulated during leaf or petal senescence and used to create a microarray, together with 91 senescence-related Arabidopsis probes. Several microarray hybridisation classes were observed demonstrating similarities and differences in gene expression profiles of these two organs. Putative functions were ascribed to 170 sequenced DNA fragments from the libraries. Notable similarities between leaf and petal senescence include a large proportion of remobilisation related genes, such as the cysteine protease gene SAG12 that were up-regulated in both tissues with age. Interesting differences included the up-regulation of chitinase and glutathione S transferase genes (GSTs) in senescing petals whilst their expression remained constant or fell with age in leaves. Semi-quantitative RT-PCR of selected genes from the SSH libraries revealed more complex patterns of expression compared to the array data.






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