Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on May 23, 2008; 10.1104/pp.108.121087

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Received April 11, 2008
Accepted May 19, 2008

THE HIGH LIGHT-INDUCIBLE POLYPEPTIDES (HLIP) STABILIZE TRIMERIC PHOTOSYSTEM I COMPLEX UNDER HIGH LIGHT CONDITIONS IN SYNECHOCYSTIS PCC 6803

Qiang Wang , Saowarath Jantaro , Bingshe Lu , Waqar Majeed , Marian Bailey , and Qingfang He *

Department of Applied Science, University of Arkansas, Little Rock, AR 72204, USA

* Corresponding author; email: qfhe{at}ualr.edu.

The high light-inducible polypeptides (HLIPs) are critical for survival under high light (HL) conditions in Synechocystis PCC 6803. In this report, we determined the localization of all four HLIPs in thylakoid protein complexes and examined effects of hli gene deletion on the photosynthetic protein complexes. The HliA and HliB proteins were found to be associated with trimeric photosystem (PS) I complexes and the Slr1128 protein, whereas HliC was associated with PsaL and TMP14. The HliD was associated with partially dissociated PS I complexes. The PS I activities of the hli mutants were 3-4 fold lower than that of wild type. The hli single mutants lost more than 30% of the PS I trimers after they were incubated in intermediate HL for 12 h. The reduction of PS I trimers were further augmented in these cells by the increase of light intensity. The quadruple hli deletion mutant contained less than half of PS I trimers following 12-h incubation in intermediate HL. It lost essentially all of the PS I trimers upon exposure to HL for 12 h. Furthermore, a mutant lacking both PS I trimers and Slr1128 showed growth defects similar to that of the quadruple hli deletion mutant under different light conditions. These results suggest that the HLIPs stabilize PS I trimers, interact with Slr1128, and protect cells under high light conditions.






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