Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 23, 2008; 10.1104/pp.108.121301

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
147/3/1126    most recent
pp.108.121301v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hou, X.
Right arrow Articles by Yu, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hou, X.
Right arrow Articles by Yu, H.
Agricola
Right arrow Articles by Hou, X.
Right arrow Articles by Yu, H.

Received April 16, 2008
Accepted May 20, 2008

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Xingliang Hou , Wen-Wei Hu , Lisha Shen , Li Yen Candy Lee , Zhen Tao , Jin-Hua Han , and Hao Yu *

Department of Biological Sciences, Faculty of Sciences, National University of Singapore, Singapore; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore

* Corresponding author; email: dbsyuhao{at}nus.edu.sg.

Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA de-represses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3, and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in various phytohormones signalling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified 8 genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signalling pathways.




This article has been cited by other articles:


Home page
 J Exp BotHome page
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2008
J. Exp. Bot., June 23, 2009; (2009) erp154v1.
[Full Text] [PDF]

Home page
 J Exp BotHome page
E. Mutasa-Gottgens and P. Hedden
Gibberellin as a factor in floral regulatory networks
J. Exp. Bot., May 1, 2009; 60(7): 1979 - 1989.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Achard and P. Genschik
Releasing the brakes of plant growth: how GAs shutdown DELLA proteins
J. Exp. Bot., March 1, 2009; 60(4): 1085 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Li, A. Lauri, M. Ziemann, A. Busch, M. Bhave, and S. Zachgo
Nuclear Activity of ROXY1, a Glutaredoxin Interacting with TGA Factors, Is Required for Petal Development in Arabidopsis thaliana
PLANT CELL, February 1, 2009; 21(2): 429 - 441.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists