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Published on May 23, 2008; 10.1104/pp.108.121301

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Received April 16, 2008
Accepted May 20, 2008

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Xingliang Hou , Wen-Wei Hu , Lisha Shen , Li Yen Candy Lee , Zhen Tao , Jin-Hua Han , and Hao Yu *

Department of Biological Sciences, Faculty of Sciences, National University of Singapore, Singapore; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore

* Corresponding author; email: dbsyuhao{at}nus.edu.sg.

Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA de-represses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3, and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in various phytohormones signalling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified 8 genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signalling pathways.




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P. Achard and P. Genschik
Releasing the brakes of plant growth: how GAs shutdown DELLA proteins
J. Exp. Bot., November 28, 2008; (2008) ern301v1.
[Abstract] [Full Text] [PDF]


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