Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on June 11, 2008; 10.1104/pp.108.121533

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
147/4/1750    most recent
pp.108.121533v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaneda, M.
Right arrow Articles by Samuels, A.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaneda, M.
Right arrow Articles by Samuels, A.L.
Agricola
Right arrow Articles by Kaneda, M.
Right arrow Articles by Samuels, A.L.

Received April 25, 2008
Accepted May 18, 2008

Tracking Monolignols During Wood Development in Pinus contorta var. latifolia

M. Kaneda , K.H. Rensing , J.C.T. Wong , B. Banno , S.D. Mansfield , and A.L. Samuels *

Department of Botany, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4; Department of Wood Sciences, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4

* Corresponding author; email: lsamuels{at}interchange.ubc.ca.

Secondary xylem (wood) formation in gymnosperms requires that the tracheid protoplasts first build an elaborate secondary cell wall from an array of polysaccharides, then reinforce it with lignin, an amorphous, three-dimensional product of the random radical coupling of monolignols. The objective of this study was to track the spatial distribution of monolignols during development as they move from symplasm to apoplasm. This was done by feeding 3H-phenylalanine to dissected cambium/developing wood from Pinus contorta var. latifolia seedlings, allowing uptake and metabolism, then rapidly freezing the cells and performing autoradiography to detect the locations of the monolignols responsible for lignification. Parallel experiments showed radioactivity was incorporated into polymeric lignin and a methanol soluble pool that was characterized by HPLC. 3H-phenylalanine was incorporated into expected lignin precursors, such as coniferyl alcohol and para-coumaryl alcohol, as well as pinoresinol. Coniferin, the glucoside of coniferyl alcohol, was detected by HPLC but was not radioactively labeled. With light microscopy, radiolabeled phenylpropanoids were detected in the rays as well as tracheids, with the two cell types showing differential sensitivity to inhibitors of protein translation and phenylpropanoid metabolism. Secondary cell walls of developing tracheids were heavily labeled when incubated with 3H-phenylalanine. Inside the cell, cytoplasm was most strongly labeled followed by Golgi and low vacuole label. Inhibitor studies suggest that the Golgi signal could be attributed to protein, rather than phenylpropanoid origins. These data, produced with the best microscopy tools that are available today, support a model where unknown membrane transporters, rather than Golgi vesicles, export monolignols.




This article has been cited by other articles:


Home page
 J Exp BotHome page
K. Marjamaa, E. M. Kukkola, and K. V. Fagerstedt
The role of xylem class III peroxidases in lignification
J. Exp. Bot., February 1, 2009; 60(2): 367 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Y. Cheung and S. C. de Vries
Membrane Trafficking: Intracellular Highways and Country Roads
Plant Physiology, August 1, 2008; 147(4): 1451 - 1453.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists