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Published on May 30, 2008; 10.1104/pp.108.121590

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Received April 22, 2008
Accepted May 2, 2008

The Arabidopsis phosphatidylinositol-3-kinase is important for pollen development

Yuree Lee , Eun-Sook Kim , Yunjung Choi , Inwhan Hwang , Christopher J. Staiger , Yong-Yoon Chung , and Youngsook Lee *

POSTECH-UZH Global Research Lab, Division of Molecular Life Sciences, POSTECH, Pohang, 790-784, Korea; Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea; School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea; Center for Plant Intracellular Trafficking, POSTECH, Pohang, 790-784, Korea; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA

* Corresponding author; email: ylee{at}postech.ac.kr.

Phosphatidylinositol 3-kinase (PI3K) has been reported to be important for normal plant growth. To characterize the role of the enzyme further, we attempted to isolate Arabidopsis plants that do not express the gene, but could not recover homozygous mutant plants. The progeny of VPS34/vps34 heterozygous plants, harboring a T-DNA insertion, showed a segregation ratio of 1:1:0 for wild-type, heterozygous, and homozygous mutant plants, indicating a gametophytic defect. Genetic transmission analysis showed that the abnormal segregation ratio was due to failure to transmit the mutant allele through the male gametophyte. Microscopic observation revealed that 2-fold higher proportions of pollen grains in heterozygous plants than wild type were dead, or showed reduced number of nuclei. Many mature pollen grains from the heterozygous plants contained large vacuoles even until the mature pollen stage, whereas pollen from wild-type plants contained many small vacuoles beginning from the vacuolated pollen stage, which indicated that vacuoles in many of the heterozygous mutant pollen did not undergo normal fission after the first mitotic division. Taken together, our results suggest that PI3K is essential for vacuole reorganization and nuclear division during pollen development.




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[Abstract] [Full Text] [PDF]


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