Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 19, 2008; 10.1104/pp.108.121913

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
148/3/1283    most recent
pp.108.121913v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rajangam, A. S.
Right arrow Articles by Teeri, T. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rajangam, A. S.
Right arrow Articles by Teeri, T. T.
Agricola
Right arrow Articles by Rajangam, A. S.
Right arrow Articles by Teeri, T. T.

Received April 23, 2008
Accepted September 16, 2008

MAP20, a microtubule-associated protein in the secondary cell walls of Populus tremula L. x tremuloides Michx is a target of the cellulose synthesis inhibitor, 2,6-dichlorobenzonitrile

Alex S. Rajangam , Manoj Kumar , Henrik Aspeborg , Gea Guerriero , Lars Arvestad , Podjamas Pansri , Christian J.-L. Brown , Sophia Hober , Kristina Blomqvist , Christina Divne , Ines Ezcurra , Ewa Mellerowicz , Bjorn Sundberg , Vincent Bulone , and Tuula T. Teeri *

KTH Biotechnology, Swedish Center of Biomimetic Fiber Engineering, AlbaNova, SE-106 91, Stockholm, Sweden; Umea Plant Science Center, Department of Forest Genetics and Plant Physiology, SE–90183 Umea, Sweden; Stockholm Bioinformatics Center, Royal Institute of Technology, AlbaNova, SE–10691 Stockholm, Sweden

* Corresponding author; email: Tuula{at}biotech.kth.se.

We have identified a gene, denoted PttMAP20, which is strongly upregulated during secondary cell wall synthesis and tightly co-regulated with the secondary wall associated CESA genes in hybrid aspen, Populus tremula L. x tremuloides Michx. Immunolocalization studies with affinity purified antibodies specific for PttMAP20 revealed that the protein is found in all cell types in developing xylem and that it is most abundant in cells forming secondary cell walls. This PttMAP20 protein sequence contains a highly conserved TPX2 domain first identified in a microtubule-associated protein in Xenopus. Overexpression of PttMAP20 in Arabidopsis leads to helical twisting of epidermal cells, frequently associated with microtubule associated proteins. In addition, a PttMAP20-YFP fusion protein expressed in tobacco leaves localizes to microtubules in leaf epidermal pavement cells. Recombinant PttMAP20 expressed in E. coli also binds specifically to in vitro assembled, taxol stabilized bovine microtubules. Finally, the herbicide 2,6-dichlorobenzonitrile (DCB), which inhibits cellulose synthesis in plants, was found to bind specifically to PttMAP20. Together with the known function of cortical microtubules in orienting cellulose microfibrils, these observations suggest that PttMAP20 has a role in cellulose biosynthesis.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists