Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 20, 2008; 10.1104/pp.108.122275

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
148/2/1082    most recent
pp.108.122275v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zubo, Y. O.
Right arrow Articles by Borner, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zubo, Y. O.
Right arrow Articles by Borner, T.
Agricola
Right arrow Articles by Zubo, Y. O.
Right arrow Articles by Borner, T.

Received April 30, 2008
Accepted August 11, 2008

Cytokinin stimulates chloroplast transcription in detached barley leaves

Yan O. Zubo , Maria V. Yamburenko , Svetlana Yu. Selivankina , Farida M. Shakirova , Azamat M. Avalbaev , Natalia V. Kudryakova , Natalia K. Zubkova , Karsten Liere , Olga N. Kulaeva , Victor V. Kusnetsov , and Thomas Borner *

Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow, 127276 Russia; Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054 Russia; Institute of Biology, Humboldt University, Chausseestrasse 117, D-10115, Berlin, Germany

* Corresponding author; email: thomas.boerner{at}rz.hu-berlin.de.

Chloroplasts are among the main targets of cytokinin action in the plant cell. We report here on the activation of transcription by cytokinin as detected by run-on assays with chloroplasts isolated from apical parts of first leaves detached from 9-day-old barley (Hordeum vulgare L.) seedlings and incubated for 3 h on a 2.2 x 10-5M solution of benzyladenine (BA). Northern analysis detected also a BA-induced increase in the accumulation of chloroplast mRNAs. A prerequisite for BA activation of chloroplast transcription was pre-incubation of leaves for 24 h on water in the light resulting in a decreased chloroplast transcription and a drastic accumulation of abscisic acid. Cytokinin enhanced transcription of several chloroplast genes above the initial level measured before BA treatment, in case of rrn16 and petD even before pre-incubation. Cytokinin effects on basal (youngest), middle, and apical (oldest) segments of primary leaves detached from plants of different ages revealed an age dependence of chloroplast gene response to BA. BA-induced stimulation of transcription of rrn16, rrn23, rps4, rps16, rbcL, atpB, and ndhC required light during the period of pre-incubation and was further enhanced by light during the incubation on BA, whereas activation of transcription of trnEY, rps14, rpl16, matK, petD and petLG depended on light during both periods. Our data revealed positive and differential effects of cytokinin on the transcription of chloroplast genes that were dependent on light and on the age (developmental stage) of cells and leaves.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists