Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 23, 2008; 10.1104/pp.108.122465

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
147/3/1251    most recent
pp.108.122465v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishizawa, A.
Right arrow Articles by Shigeoka, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishizawa, A.
Right arrow Articles by Shigeoka, S.
Agricola
Right arrow Articles by Nishizawa, A.
Right arrow Articles by Shigeoka, S.

Received May 2, 2008
Accepted May 20, 2008

Galactinol and raffinose as a novel function to protect plants from oxidative damage

Ayako Nishizawa , Yukinori Yabuta , and Shigeru Shigeoka *

Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan

* Corresponding author; email: shigeoka{at}nara.kindai.ac.jp.

Galactinol synthase (GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides that function as osmoprotectants in plant cells. In leaves of Arabidopsis plants overexpressing heat shock transcription factor A2 (HsfA2), the transcription of GolS1, 2 and 4 and raffinose synthase (RS) 2 was highly induced and thus levels of galactinol and raffinose increased compared with those in wild-type plants under control growth conditions. In leaves of the wild-type plants, treatment with 50 µM methylviologen (MV) increased the transcript levels of not only HsfA2, but also GolS1, 2, 3, 4 and 8 and RS2, 4, 5 and 6, the total activities of GolS isoenzymes, and the levels of galactinol and raffinose. GolS1 or GolS2-overexpressing Arabidopsis plants (Ox-GolS1-11, Ox-GolS2-8, and Ox-GolS2-29) had increased levels of galactinol and raffinose in the leaves compared with the wild-type plants under control growth conditions. High intracellular levels of galactinol and raffinose in the transgenic plants were correlated with increased tolerance to MV treatment and salinity or chilling stress. Galactinol and raffinose effectively protected salicylate from attack by hydroxyl radicals in vitro. The present findings suggest the possibility that galactinol and raffinose scavenge hydroxyl radicals as a novel function to protect plant cells from oxidative damage caused by MV treatment, salinity or chilling.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
K. Maruyama, M. Takeda, S. Kidokoro, K. Yamada, Y. Sakuma, K. Urano, M. Fujita, K. Yoshiwara, S. Matsukura, Y. Morishita, et al.
Metabolic Pathways Involved in Cold Acclimation Identified by Integrated Analysis of Metabolites and Transcripts Regulated by DREB1A and DREB2A
Plant Physiology, August 1, 2009; 150(4): 1972 - 1980.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. Van den Ende and R. Valluru
Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging?
J. Exp. Bot., January 1, 2009; 60(1): 9 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Wu, Z. Zhang, H. Zhang, X.-C. Wang, and R. Huang
Transcriptional Modulation of Ethylene Response Factor Protein JERF3 in the Oxidative Stress Response Enhances Tolerance of Tobacco Seedlings to Salt, Drought, and Freezing
Plant Physiology, December 1, 2008; 148(4): 1953 - 1963.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists