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Published on September 3, 2008; 10.1104/pp.108.123026

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Received May 15, 2008
Accepted August 11, 2008

{beta} subunits of the SnRK1 complexes share a common ancestral function together with expression and function specificities; physical interaction with nitrate reductase specifically occurs via AKIN{beta}1 subunit

C. Polge , M. Jossier , P. Crozet , L. Gissot , and M. Thomas *

Laboratoire Signalisation et Regulation Coordonnee du Metabolisme Carbone et Azote, Institut de Biotechnologie des Plantes (UMR8618), Universite Paris-Sud, F-91405 Orsay Cedex, France

* Corresponding author; email: martine.thomas{at}u-psud.fr.

The SNF1/AMPK/SnRK1 kinases are evolutionary conserved kinases involved in yeast, mammals and plants in the control of energy balance. This heterotrimeric enzyme is composed of one {alpha}-type catalytic subunit and two {gamma}- and {beta}-type regulatory subunits. In yeast it has been proposed that the {beta}-type subunits regulate both the localization of the kinase complexes within the cell and the interaction of the kinase with its targets. In the present work, we demonstrate that the three {beta}-type subunits of Arabidopsis thaliana (AKIN{beta}1, {beta}2 and {beta}3) restore the growth phenotype of the yeast sip1{Delta}sip2{Delta}gal83{Delta} triple mutant thus suggesting the conservation of an ancestral function. Expression analyses, using AKIN{beta} promoter::GUS transgenic lines, reveal different and specific patterns of expression for each subunit according to organs, developmental stages and environmental conditions. Finally our results show that the {beta}-type subunits are involved in the specificity of interaction of the kinase with the cytosolic nitrate reductase (NR). Together with previous cell-free phosphorylation data, they strongly support the proposal that NR is a real target of SnRK1 in the physiological context. Altogether our data suggest the conservation of (an) ancestral basic function(s) together with specialized functions for each {beta}-type subunit in plants.




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