Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 20, 2008; 10.1104/pp.108.123075

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
148/2/829    most recent
pp.108.123075v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oikawa, K.
Right arrow Articles by Wada, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oikawa, K.
Right arrow Articles by Wada, M.
Agricola
Right arrow Articles by Oikawa, K.
Right arrow Articles by Wada, M.

Received May 19, 2008
Accepted August 4, 2008

Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement

Kazusato Oikawa , Akihiro Yamasato , Sam-Geun Kong , Masahiro Kasahara , Masato Nakai , Fumio Takahashi , Yasunobu Ogura , Takatoshi Kagawa , and Masamitsu Wada *

National Institute for Basic Biology, Okazaki, Aichi444-8585, Japan; Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Nojihigashi, Kusatsu, Shiga 525-8577, Japan; Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan

* Corresponding author; email: wada{at}nibb.ac.jp.

Chloroplasts change their intracellular distribution in response to light intensity. Previously, we isolated the chloroplast unusual positioning (chup1) mutant of Arabidopsis thaliana (Oikawa et al., 2003). This mutant is defective in normal chloroplast relocation movement and shows aggregation of chloroplasts at the bottom of palisade mesophyll cells. The isolated gene encodes a protein with an actin-binding motif. Here, we used biochemical analyses to determine the subcellular localization of full-length CHUP1 on the chloroplast outer envelope. A CHUP1-GFP fusion, which was detected at the outermost part of mesophyll cell chloroplasts, complemented the chup1 phenotype, but GFP-CHUP1, which localized mainly in the cytosol, did not. Overexpression of the N-terminal hydrophobic region (NtHR) of CHUP1 fused with GFP (NtHR-GFP) induced a chup1-like phenotype, indicating a dominant-negative effect on chloroplast relocation movement. A similar pattern was found in chloroplast outer envelope protein 7 (OEP7)-GFP transformants, and a protein containing OEP7 in place of NtHR complemented the mutant phenotype. Physiological analyses of transgenic Arabidopsis plants expressing truncated CHUP1 in a chup1-mutant background and cytoskeletal inhibitor experiments showed that the coiled-coil region of CHUP1 anchors chloroplasts firmly on the plasma membrane, consistent with the localization of coiled-coil-GFP on the plasma membrane. Thus, CHUP1 localization on chloroplasts, with the N-terminus inserted into the chloroplast outer envelope and the C-terminus facing the cytosol is essential for CHUP1 function, and the coiled-coil region of CHUP1 prevents chloroplast aggregation and participates in chloroplast relocation movement.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Kadota, N. Yamada, N. Suetsugu, M. Hirose, C. Saito, K. Shoda, S. Ichikawa, T. Kagawa, A. Nakano, and M. Wada
Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis
PNAS, August 4, 2009; 106(31): 13106 - 13111.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Takagi, H. Takamatsu, and N. Sakurai-Ozato
Chloroplast anchoring: its implications for the regulation of intracellular chloroplast distribution
J. Exp. Bot., August 1, 2009; 60(12): 3301 - 3310.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Jouhet and J. C. Gray
Interaction of Actin and the Chloroplast Protein Import Apparatus
J. Biol. Chem., July 10, 2009; 284(28): 19132 - 19141.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists