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Published on July 3, 2008; 10.1104/pp.108.123141

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Received May 19, 2008
Accepted June 20, 2008

Proteome-wide characterization of seed aging in Arabidopsis. A comparison between artificial and natural aging protocols

Loic Rajjou *, Yoann Lovigny , Steven PC Groot , Maya Belghazi , Claudette Job , and Dominique Job

Unite Mixte de Recherche 204 Institut National de la Recherche Agronomique-AgroParisTech "Laboratoire de Biologie des Semences", AgroParisTech, Chaire de Physiologie Vegetale, 16 rue Claude Bernard, F-75231 Paris Cedex 05, France; Unite Mixte de Recherche 204 Institut National de la Recherche Agronomique-AgroParisTech "Laboratoire de Biologie des Semences", Institut Jean-Pierre Bourgin-Institut National de la Recherche Agronomique, Route de St-Cyr, F-78026 Versailles Cedex, France; Centre d'Analyse Proteomique de Marseille, IFR Jean Roche - 51, boulevard Pierre Dramard, F-13916 Marseille Cedex 20, France; Plant Research International, Wageningen University and Research centre, P.O. Box 16, 6700 AA Wageningen, The Netherlands; Unite Mixte de Recherche 5240 Centre National de la Recherche Scientifique-Universite Claude Bernard Lyon-Institut National des Sciences Appliquees-Bayer CropScience Joint Laboratory, Bayer CropScience, 14-20 rue Pierre Baizet - F-69263 Lyon Cedex 9, France

* Corresponding author; email: loic.rajjou{at}agroparistech.fr.

A variety of mechanisms has been proposed to account for the extension of life span in seeds (seed longevity). In the present work, we have used Arabidopsis thaliana seeds as a model and carried out differential proteomics to investigate this trait, which is of both ecological and agricultural importance. In our system based on a controlled deterioration treatment (CDT), we compared seed samples treated for different periods of time, up to seven days. Germination tests showed a progressive decrease of germination vigor depending on the duration of CDT. Proteomic analyses revealed that this loss in seed vigor can be accounted for by protein changes in the dry seeds and by an inability of the low-vigor seeds to display a normal proteome during germination. Furthermore, the CDT strongly increased the extent of protein oxidation (carbonylation), which might induce a loss of functional properties of seed proteins and enzymes and/or enhance their susceptibility toward proteolysis. These results unveiled essential mechanisms for seed vigor as translational capacity, mobilization of seed storage reserves and detoxification efficiency. Finally, the present work shows that similar molecular events accompany artificial and natural seed aging.




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[Abstract] [Full Text] [PDF]


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